User:Alisha, Deepa, Pamiz/Sandbox 1: Difference between revisions

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Inorganic Pi produced from the hydrolysis of ATP drives a conformational change in the enzyme and allows release of H+ into the highly acidic environment.5 The enzyme catalyzes this reaction by changing conformation states between E1 and E2 (Scheme 1).6 [[Image:e1 to e2.gif|300px|right|thumb| The reaction begins when a hydronium ion binds to the enzyme on the cytoplasmic surface.6 MgATP will phosphorylate the enzyme at an Asp386 residue in a DKTG amino acid sequence to form the E1~Pi H+ intermediate.6 E1 undergoes a conformational change to form E2, where the ion site is exposed and H+ is released at a pH ~ 1.0.7 Extracellular K+ ions then bind to the same exposed region and the enzyme dephosphorylates.7 An occluded form of the enzyme (trapped) is formed once K+ ions bind; the enzyme de-occludes, reforms the E1 complex, and K+ is released.7]] H+/K+-ATPase pumps are normally inactive and inside vesicles.9 These vesicles are activated through a signaling pathway.

H+/K+-ATPase signal pathway (acetylcholine, histamine, and gastrin) activates the pump in order to move the vesicles toward the lumen.9 These signals bind to their corresponding receptors and activate the cAMP and Ca2+ dependent pathways.9 Increased levels of intracellular Ca2+ and cAMP will promote the translocation of vesicles to the canalicular membrane, activating the H+/K+-ATPase.9 Histamine binds to Histamine H2, and sends a signal through a G protein which activates adenylate cyclase, and catalyzes the conversion of ATP to cAMP.9 Gastrin will stimulate the release of histamine by binding to CCK2.9 Acetylcholine binds to Muscarinic M3 and releases Ca2+ from the endoplasmic reticulum.9

Revision as of 22:29, 6 December 2013 view source Deepa Patel (talk \| contribs) 230 edits →‎Conformational Change and Signaling Pathway of H+/K+-ATPase ← Older edit		Revision as of 22:29, 6 December 2013 view source Deepa Patel (talk \| contribs) 230 edits →‎Conformational Change and Signaling Pathway of H+/K+-ATPase Newer edit →
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	Inorganic Pi produced from the hydrolysis of ATP drives a conformational change in the enzyme and allows release of H+ into the highly acidic environment.5 The enzyme catalyzes this reaction by changing conformation states between E1 and E2 (Scheme 1).6 [[Image:e1 to e2.gif\|300px\|right\|thumb\| The reaction begins when a hydronium ion binds to the enzyme on the cytoplasmic surface.6 MgATP will phosphorylate the enzyme at an Asp386 residue in a DKTG amino acid sequence to form the E1~Pi H+ intermediate.6 E1 undergoes a conformational change to form E2, where the ion site is exposed and H+ is released at a pH ~ 1.0.7 Extracellular K+ ions then bind to the same exposed region and the enzyme dephosphorylates.7 An occluded form of the enzyme (trapped) is formed once K+ ions bind; the enzyme de-occludes, reforms the E1 complex, and K+ is released.7]] H+/K+-ATPase pumps are normally inactive and inside vesicles.9 These vesicles are activated through a signaling pathway.		Inorganic Pi produced from the hydrolysis of ATP drives a conformational change in the enzyme and allows release of H+ into the highly acidic environment.5 The enzyme catalyzes this reaction by changing conformation states between E1 and E2 (Scheme 1).6 [[Image:e1 to e2.gif\|300px\|right\|thumb\| The reaction begins when a hydronium ion binds to the enzyme on the cytoplasmic surface.6 MgATP will phosphorylate the enzyme at an Asp386 residue in a DKTG amino acid sequence to form the E1~Pi H+ intermediate.6 E1 undergoes a conformational change to form E2, where the ion site is exposed and H+ is released at a pH ~ 1.0.7 Extracellular K+ ions then bind to the same exposed region and the enzyme dephosphorylates.7 An occluded form of the enzyme (trapped) is formed once K+ ions bind; the enzyme de-occludes, reforms the E1 complex, and K+ is released.7]] H+/K+-ATPase pumps are normally inactive and inside vesicles.9 These vesicles are activated through a signaling pathway.


	H+/K+-ATPase signal pathway (acetylcholine, histamine, and gastrin) activates the pump in order to move the vesicles toward the lumen.9 These signals bind to their corresponding receptors and activate the cAMP and Ca2+ dependent pathways.9 Increased levels of intracellular Ca2+ and cAMP will promote the translocation of vesicles to the canalicular membrane, activating the H+/K+-ATPase.9 Histamine binds to Histamine H2, and sends a signal through a G protein which activates adenylate cyclase, and catalyzes the conversion of ATP to cAMP.9 Gastrin will stimulate the release of histamine by binding to CCK2.9 Acetylcholine binds to Muscarinic M3 and releases Ca2+ from the endoplasmic reticulum.9		H+/K+-ATPase signal pathway (acetylcholine, histamine, and gastrin) activates the pump in order to move the vesicles toward the lumen.9 These signals bind to their corresponding receptors and activate the cAMP and Ca2+ dependent pathways.9 Increased levels of intracellular Ca2+ and cAMP will promote the translocation of vesicles to the canalicular membrane, activating the H+/K+-ATPase.9 Histamine binds to Histamine H2, and sends a signal through a G protein which activates adenylate cyclase, and catalyzes the conversion of ATP to cAMP.9 Gastrin will stimulate the release of histamine by binding to CCK2.9 Acetylcholine binds to Muscarinic M3 and releases Ca2+ from the endoplasmic reticulum.9