2qqm: Difference between revisions

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Revision as of 19:28, 20 March 2008

File:2qqm.jpg

, resolution 2.000Å

Ligands:

and

Gene:

NRP1, NRP, VEGF165R (Homo sapiens)

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of the a2b1b2 Domains from Human Neuropilin-1

OverviewOverview

Neuropilins (Nrps) are co-receptors for class 3 semaphorins and vascular endothelial growth factors and important for the development of the nervous system and the vasculature. The extracellular portion of Nrp is composed of two domains that are essential for semaphorin binding (a1a2), two domains necessary for VEGF binding (b1b2), and one domain critical for receptor dimerization (c). We report several crystal structures of Nrp1 and Nrp2 fragments alone and in complex with antibodies that selectively block either semaphorin or vascular endothelial growth factor (VEGF) binding. In these structures, Nrps adopt an unexpected domain arrangement in which the a2, b1, and b2 domains form a tightly packed core that is only loosely connected to the a1 domain. The locations of the antibody epitopes together with in vitro experiments indicate that VEGF and semaphorin do not directly compete for Nrp binding. Based upon our structural and functional data, we propose possible models for ligand binding to neuropilins.

About this StructureAbout this Structure

2QQM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding., Appleton BA, Wu P, Maloney J, Yin J, Liang WC, Stawicki S, Mortara K, Bowman KK, Elliott JM, Desmarais W, Bazan JF, Bagri A, Tessier-Lavigne M, Koch AW, Wu Y, Watts RJ, Wiesmann C, EMBO J. 2007 Nov 28;26(23):4902-12. Epub 2007 Nov 8. PMID:17989695

Page seeded by OCA on Thu Mar 20 18:28:34 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2qqm.jpg|left|200px]]<br /><applet load="2qqm" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2qqm.jpg|left|200px]]
-caption="2qqm, resolution 2.000&Aring;" />
-'''Crystal Structure of the a2b1b2 Domains from Human Neuropilin-1'''<br />
+ {{Structure
+|PDB= 2qqm |SIZE=350|CAPTION= <scene name='initialview01'>2qqm</scene>, resolution 2.000&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
+|ACTIVITY=
+|GENE= NRP1, NRP, VEGF165R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''Crystal Structure of the a2b1b2 Domains from Human Neuropilin-1'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-QQM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QQM OCA].
+QQM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QQM OCA].
 ==Reference==
-Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding., Appleton BA, Wu P, Maloney J, Yin J, Liang WC, Stawicki S, Mortara K, Bowman KK, Elliott JM, Desmarais W, Bazan JF, Bagri A, Tessier-Lavigne M, Koch AW, Wu Y, Watts RJ, Wiesmann C, EMBO J. 2007 Nov 28;26(23):4902-12. Epub 2007 Nov 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17989695 17989695]
+Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding., Appleton BA, Wu P, Maloney J, Yin J, Liang WC, Stawicki S, Mortara K, Bowman KK, Elliott JM, Desmarais W, Bazan JF, Bagri A, Tessier-Lavigne M, Koch AW, Wu Y, Watts RJ, Wiesmann C, EMBO J. 2007 Nov 28;26(23):4902-12. Epub 2007 Nov 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17989695 17989695]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 34: / Line 43: @@
 [[Category: vegf receptor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:28:34 2008''