2xrm: Difference between revisions
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==Processed Intracellular subtilisin from B. clausii== | |||
=== | <StructureSection load='2xrm' size='340' side='right' caption='[[2xrm]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2xrm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700160 Atcc 700160]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XRM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XRM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x8j|2x8j]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xrm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xrm RCSB], [http://www.ebi.ac.uk/pdbsum/2xrm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A distinct class of the biologically important subtilisin family of serine proteases functions exclusively within the cell and forms a major component of the bacilli degradome. However, the mode and mechanism of posttranslational regulation of intracellular protease activity are unknown. Here we describe the role played by a short N-terminal extension prosequence novel amongst the subtilisins that regulates intracellular subtilisin protease (ISP) activity through two distinct modes: active site blocking and catalytic triad rearrangement. The full-length proenzyme (proISP) is inactive until specific proteolytic processing removes the first 18 amino acids that comprise the N-terminal extension, with processing appearing to be performed by ISP itself. A synthetic peptide corresponding to the N-terminal extension behaves as a mixed noncompetitive inhibitor of active ISP with a K(i) of 1 muM. The structure of the processed form has been determined at 2.6 A resolution and compared with that of the full-length protein, in which the N-terminal extension binds back over the active site. Unique to ISP, a conserved proline introduces a backbone kink that shifts the scissile bond beyond reach of the catalytic serine and in addition the catalytic triad is disrupted. In the processed form, access to the active site is unblocked by removal of the N-terminal extension and the catalytic triad rearranges to a functional conformation. These studies provide a new molecular insight concerning the mechanisms by which subtilisins and protease activity as a whole, especially within the confines of a cell, can be regulated. | |||
Regulation of an intracellular subtilisin protease activity by a short propeptide sequence through an original combined dual mechanism.,Gamble M, Kunze G, Dodson EJ, Wilson KS, Jones DD Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3536-41. Epub 2011 Feb 9. PMID:21307308<ref>PMID:21307308</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Subtilisin|Subtilisin]] | *[[Subtilisin|Subtilisin]] | ||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Atcc 700160]] | [[Category: Atcc 700160]] | ||
[[Category: Subtilisin]] | [[Category: Subtilisin]] | ||
[[Category: Dodson, E J | [[Category: Dodson, E J]] | ||
[[Category: Gamble, M | [[Category: Gamble, M]] | ||
[[Category: Jones, D D | [[Category: Jones, D D]] | ||
[[Category: Kunze, G | [[Category: Kunze, G]] | ||
[[Category: Wilson, K S | [[Category: Wilson, K S]] | ||
[[Category: Activated form]] | [[Category: Activated form]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Post-translational modification]] | [[Category: Post-translational modification]] | ||