2q9e: Difference between revisions

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Revision as of 19:23, 20 March 2008

File:2q9e.jpg

, resolution 2.100Å

Sites:

, and

Ligands:

Gene:

E (Pseudomonas phage D3112)

Activity:

Lysozyme, with EC number 3.2.1.17

Coordinates:

save as pdb, mmCIF, xml

Structure of spin-labeled T4 lysozyme mutant S44R1

OverviewOverview

Site-directed spin labeling provides a means for exploring structure and dynamics in proteins. To interpret the complex EPR spectra that often arise, it is necessary to characterize the rotamers of the spin-labeled side chain and the interactions they make with the local environment in proteins of known structure. For this purpose, crystal structures have been determined for T4 lysozyme bearing a nitroxide side chain (R1) at the solvent-exposed helical sites 41 and 44 in the B helix. These sites are of particular interest in that the corresponding EPR spectra reveal two dynamic states of R1, one of which is relatively immobilized suggesting interactions of the nitroxide with the environment. The crystal structures together with the effect of mutagenesis of nearest neighbors on the motion of R1 suggest intrahelical interactions of 41R1 with the i + 4 residue and of 44R1 with the i + 1 residue. Such interactions appear to be specific to particular rotamers of the R1 side chain.

About this StructureAbout this Structure

2Q9E is a Single protein structure of sequence from Pseudomonas phage d3112. Full crystallographic information is available from OCA.

ReferenceReference

Structural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme., Guo Z, Cascio D, Hideg K, Hubbell WL, Protein Sci. 2008 Feb;17(2):228-39. Epub 2007 Dec 20. PMID:18096642

Page seeded by OCA on Thu Mar 20 18:23:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2q9e.jpg|left|200px]]<br /><applet load="2q9e" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2q9e.jpg|left|200px]]
-caption="2q9e, resolution 2.100&Aring;" />
-'''Structure of spin-labeled T4 lysozyme mutant S44R1'''<br />
+ {{Structure
+|PDB= 2q9e |SIZE=350|CAPTION= <scene name='initialview01'>2q9e</scene>, resolution 2.100&Aring;
+|SITE= <scene name='pdbsite=AC1:Hed+Binding+Site+For+Residue+A+900'>AC1</scene>, <scene name='pdbsite=AC2:Hed+Binding+Site+For+Residue+B+910'>AC2</scene> and <scene name='pdbsite=AC3:Hed+Binding+Site+For+Residue+B+920'>AC3</scene>
+|LIGAND= <scene name='pdbligand=HED:2-HYDROXYETHYL DISULFIDE'>HED</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
+|GENE= E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10708 Pseudomonas phage D3112])
+}}
+'''Structure of spin-labeled T4 lysozyme mutant S44R1'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-Q9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_d3112 Pseudomonas phage d3112] with <scene name='pdbligand=HED:'>HED</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Sites: <scene name='pdbsite=AC1:Hed+Binding+Site+For+Residue+A+900'>AC1</scene>, <scene name='pdbsite=AC2:Hed+Binding+Site+For+Residue+B+910'>AC2</scene> and <scene name='pdbsite=AC3:Hed+Binding+Site+For+Residue+B+920'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q9E OCA].
+Q9E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_d3112 Pseudomonas phage d3112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q9E OCA].
 ==Reference==
-Structural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme., Guo Z, Cascio D, Hideg K, Hubbell WL, Protein Sci. 2008 Feb;17(2):228-39. Epub 2007 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18096642 18096642]
+Structural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme., Guo Z, Cascio D, Hideg K, Hubbell WL, Protein Sci. 2008 Feb;17(2):228-39. Epub 2007 Dec 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18096642 18096642]
 [[Category: Lysozyme]]
 [[Category: Pseudomonas phage d3112]]
@@ Line 26: / Line 35: @@
 [[Category: t4 lysozyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:37:24 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:23:20 2008''