4heb: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{STRUCTURE_4heb|  PDB=4heb  |  SCENE=  }}
+==The Crystal structure of Maf protein of Bacillus subtilis==
-===The Crystal structure of Maf protein of Bacillus subtilis===
+<StructureSection load='4heb' size='340' side='right' caption='[[4heb]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
-{{ABSTRACT_PUBMED_24210219}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4heb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HEB FirstGlance]. <br>
-==Function==
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2p5x|2p5x]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">maf, BSU28050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4heb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4heb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4heb RCSB], [http://www.ebi.ac.uk/pdbsum/4heb PDBsum]</span></td></tr>
+</table>
+== Function ==
 [[http://www.uniprot.org/uniprot/MAF_BACSU MAF_BACSU]] Involved in septum formation.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Maf (for multicopy associated filamentation) proteins represent a large family of conserved proteins implicated in cell division arrest but whose biochemical activity remains unknown. Here, we show that the prokaryotic and eukaryotic Maf proteins exhibit nucleotide pyrophosphatase activity against 5-methyl-UTP, pseudo-UTP, 5-methyl-CTP, and 7-methyl-GTP, which represent the most abundant modified bases in all organisms, as well as against canonical nucleotides dTTP, UTP, and CTP. Overexpression of the Maf protein YhdE in E. coli cells increased intracellular levels of dTMP and UMP, confirming that dTTP and UTP are the in vivo substrates of this protein. Crystal structures and site-directed mutagenesis of Maf proteins revealed the determinants of their activity and substrate specificity. Thus, pyrophosphatase activity of Maf proteins toward canonical and modified nucleotides might provide the molecular mechanism for a dual role of these proteins in cell division arrest and house cleaning.
-==About this Structure==
+Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides.,Tchigvintsev A, Tchigvintsev D, Flick R, Popovic A, Dong A, Xu X, Brown G, Lu W, Wu H, Cui H, Dombrowski L, Joo JC, Beloglazova N, Min J, Savchenko A, Caudy AA, Rabinowitz JD, Murzin AG, Yakunin AF Chem Biol. 2013 Oct 22. pii: S1074-5521(13)00347-5. doi:, 10.1016/j.chembiol.2013.09.011. PMID:24210219<ref>PMID:24210219</ref>
-[[4heb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEB OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024210219</ref><references group="xtra"/><references/>
+</div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bacillus globigii migula 1900]]
-[[Category: Arrowsmith, C H.]]
+[[Category: Arrowsmith, C H]]
-[[Category: Bountra, C.]]
+[[Category: Bountra, C]]
-[[Category: Brown, G.]]
+[[Category: Brown, G]]
-[[Category: Dombrovski, L.]]
+[[Category: Dombrovski, L]]
-[[Category: Dong, A.]]
+[[Category: Dong, A]]
-[[Category: Edwards, A M.]]
+[[Category: Edwards, A M]]
-[[Category: Flick, R.]]
+[[Category: Flick, R]]
-[[Category: Iakounine, A.]]
+[[Category: Iakounine, A]]
-[[Category: Min, J.]]
+[[Category: Min, J]]
-[[Category: SGC, Structural Genomics Consortium.]]
+[[Category: Structural genomic]]
-[[Category: Tchigvintsev, D.]]
+[[Category: Tchigvintsev, D]]
-[[Category: Wu, H.]]
+[[Category: Wu, H]]
 [[Category: Bacillus subtili]]
 [[Category: Cell cycle]]
@@ Line 29: / Line 40: @@
 [[Category: Nucleoside triphosphate pyrophosphatase]]
 [[Category: Sgc]]
-[[Category: Structural genomic]]
-[[Category: Structural genomics consortium]]