2px5: Difference between revisions

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Revision as of 19:18, 20 March 2008

File:2px5.jpg

, resolution 2.30Å

Ligands:

and

Gene:

NS5 (Murray Valley encephalitis virus)

Activity:

RNA-directed RNA polymerase, with EC number 2.7.7.48

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Orthorhombic crystal form)

OverviewOverview

We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

About this StructureAbout this Structure

2PX5 is a Single protein structure of sequence from Murray valley encephalitis virus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:17622627

Page seeded by OCA on Thu Mar 20 18:18:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2px5.jpg|left|200px]]<br /><applet load="2px5" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2px5.jpg|left|200px]]
-caption="2px5, resolution 2.30&Aring;" />
-'''Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Orthorhombic crystal form)'''<br />
+ {{Structure
+|PDB= 2px5 |SIZE=350|CAPTION= <scene name='initialview01'>2px5</scene>, resolution 2.30&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48]
+|GENE= NS5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11079 Murray Valley encephalitis virus])
+}}
+'''Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Orthorhombic crystal form)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-PX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX5 OCA].
+PX5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX5 OCA].
 ==Reference==
-Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17622627 17622627]
+Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17622627 17622627]
 [[Category: Murray valley encephalitis virus]]
 [[Category: RNA-directed RNA polymerase]]
@@ Line 32: / Line 41: @@
 [[Category: oxford protein production facility]]
 [[Category: sah]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:33:53 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:18:29 2008''