2pr6: Difference between revisions
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[[Image:2pr6.jpg|left|200px]] | [[Image:2pr6.jpg|left|200px]] | ||
'''Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Light Structure)''' | {{Structure | ||
|PDB= 2pr6 |SIZE=350|CAPTION= <scene name='initialview01'>2pr6</scene>, resolution 1.950Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=FMN:FLAVIN MONONUCLEOTIDE'>FMN</scene> | |||
|ACTIVITY= | |||
|GENE= pfyP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |||
}} | |||
'''Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Light Structure)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2PR6 is a [ | 2PR6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PR6 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA., Moglich A, Moffat K, J Mol Biol. 2007 Oct 12;373(1):112-26. Epub 2007 Aug 2. PMID:[http:// | Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA., Moglich A, Moffat K, J Mol Biol. 2007 Oct 12;373(1):112-26. Epub 2007 Aug 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17764689 17764689] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: light-oxygen-voltage]] | [[Category: light-oxygen-voltage]] | ||
[[Category: lov]] | [[Category: lov]] | ||
[[Category: | [[Category: pa]] | ||
[[Category: per-arnt-sim]] | [[Category: per-arnt-sim]] | ||
[[Category: signaling protein]] | [[Category: signaling protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:16:26 2008'' | ||
Revision as of 19:16, 20 March 2008
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| , resolution 1.950Å | |||||||
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| Ligands: | |||||||
| Gene: | pfyP (Bacillus subtilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Light Structure)
OverviewOverview
The photosensor YtvA binds flavin mononucleotide and regulates the general stress reaction in Bacillus subtilis in response to blue light illumination. It belongs to the family of light-oxygen-voltage (LOV) proteins that were first described in plant phototropins and form a subgroup of the Per-Arnt-Sim (PAS) superfamily. Here, we report the three-dimensional structure of the LOV domain of YtvA in its dark and light states. The protein assumes the global fold common to all PAS domains and dimerizes via a hydrophobic interface. Directly C-terminal to the core of the LOV domain, an alpha-helix extends into the solvent. Light absorption causes formation of a covalent bond between a conserved cysteine residue and atom C(4a) of the FMN ring, which triggers rearrangements throughout the LOV domain. Concomitantly, in the dark and light structures, the two subunits of the dimeric protein rotate relative to each other by 5 degrees . This small quaternary structural change is presumably a component of the mechanism by which the activity of YtvA is regulated in response to light. In terms of both structure and signaling mechanism, YtvA differs from plant phototropins and more closely resembles prokaryotic heme-binding PAS domains.
About this StructureAbout this Structure
2PR6 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA., Moglich A, Moffat K, J Mol Biol. 2007 Oct 12;373(1):112-26. Epub 2007 Aug 2. PMID:17764689
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