Ku protein: Difference between revisions
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== Domains == | == Domains == | ||
Consisting of | Consisting of <scene name='56/567269/Ku70_dimer/1'>four domains</scene> (α/β-Domain, β-barrel, C-terminal arm, DNA-binding ring), the Ku70 subunit dimerizes with the Ku80 subunit to form the protein.<ref name="Walker"/> | ||
Unlike other DNA binding proteins, the Ku protein is asymmetrical from the differences between the Ku70 and Ku80 subunits. | Unlike other DNA binding proteins, the Ku protein is asymmetrical from the differences between the Ku70 and Ku80 subunits. | ||
This asymmetry leads to different favorable locations for DNA based on major and minor grooves.<ref name="Walker"/> | This asymmetry leads to different favorable locations for DNA based on major and minor grooves.<ref name="Walker"/> | ||
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The <scene name='56/567269/Ku70_dimer/4'>β-barrel</scene> is the main source of interactions of the <scene name='56/567269/Ku_heterodimer/3'>Ku heterodimer</scene> itself and <scene name='56/567269/Bound_dna/3'>DNA helix</scene>, with each <scene name='56/567269/Ku70_dimer/4'>β-barrel</scene> being composed of seven β strands with the majority in antiparallel arrangement.<ref name="Walker"/> | The <scene name='56/567269/Ku70_dimer/4'>β-barrel</scene> is the main source of interactions of the <scene name='56/567269/Ku_heterodimer/3'>Ku heterodimer</scene> itself and <scene name='56/567269/Bound_dna/3'>DNA helix</scene>, with each <scene name='56/567269/Ku70_dimer/4'>β-barrel</scene> being composed of seven β strands with the majority in antiparallel arrangement.<ref name="Walker"/> | ||
The quantity of the strands lends the structures to be symmetrical. Both <scene name='56/567269/Ku70_dimer/4'>β-barrels</scene> in the dimer form the base of the cradle by fitting in the grooves of <scene name='56/567269/Bound_dna/3'>DNA</scene>. | The quantity of the strands lends the structures to be symmetrical. Both <scene name='56/567269/Ku70_dimer/4'>β-barrels</scene> in the dimer form the base of the cradle by fitting in the grooves of <scene name='56/567269/Bound_dna/3'>DNA</scene>. | ||
=== C-terminal arm === | === C-terminal arm === | ||
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The <scene name='56/567269/Ku70_dimer/7'>C-terminal arm</scene> is an α-helical domain that associates with the β-barrel of the opposite subunit, with the arm stretching across the <scene name='56/567269/Bound_dna/3'>DNA helix</scene>.<ref name="Walker"/> | The <scene name='56/567269/Ku70_dimer/7'>C-terminal arm</scene> is an α-helical domain that associates with the β-barrel of the opposite subunit, with the arm stretching across the <scene name='56/567269/Bound_dna/3'>DNA helix</scene>.<ref name="Walker"/> | ||
As a result, the <scene name='56/567269/Ku70_dimer/7'>C-terminal arm</scene> strengthens the cradle composed of the two β-barrels. | As a result, the <scene name='56/567269/Ku70_dimer/7'>C-terminal arm</scene> strengthens the cradle composed of the two β-barrels. | ||
=== DNA binding ring === | === DNA binding ring === | ||