Single stranded binding protein: Difference between revisions
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DNA, whereas modification of either lysine residues (with acetic anhydride) or tryptophan | DNA, whereas modification of either lysine residues (with acetic anhydride) or tryptophan | ||
residues (with N-bromosuccinimide) led to complete loss of binding activity <ref>PMID: 2087220</ref>. | residues (with N-bromosuccinimide) led to complete loss of binding activity <ref>PMID: 2087220</ref>. | ||
The two tryptophan residues involved in DNA binding are Trp40 and Trp54, which was | The two tryptophan residues involved in DNA binding are <scene name='56/566528/Trp_40/1'>Trp40</scene> and Trp54, which was | ||
determined by mutagenesis. One more binding site was determined by site-specific mutagenesis. | determined by mutagenesis. One more binding site was determined by site-specific mutagenesis. | ||
When His55 is substituted with Leu it decreases binding affinity. All of these residues | When His55 is substituted with Leu it decreases binding affinity. All of these residues | ||