2p8q: Difference between revisions
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'''Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain''' | {{Structure | ||
|PDB= 2p8q |SIZE=350|CAPTION= <scene name='initialview01'>2p8q</scene>, resolution 2.35Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= KPNB1, NTF97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2P8Q is a [ | 2P8Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P8Q OCA]. | ||
==Reference== | ==Reference== | ||
Molecular basis for the recognition of snurportin 1 by importin beta., Mitrousis G, Olia AS, Walker-Kopp N, Cingolani G, J Biol Chem. 2008 Jan 9;. PMID:[http:// | Molecular basis for the recognition of snurportin 1 by importin beta., Mitrousis G, Olia AS, Walker-Kopp N, Cingolani G, J Biol Chem. 2008 Jan 9;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18187419 18187419] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: snurportin]] | [[Category: snurportin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:10:09 2008'' | ||
Revision as of 19:10, 20 March 2008
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| , resolution 2.35Å | |||||||
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| Gene: | KPNB1, NTF97 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain
OverviewOverview
The nuclear import of uridine-rich ribonucleoproteins (U snRNPs) is mediated by the transport adaptor snurportin 1 (SNP1). Similar to importin alpha, SNP1 uses an N-terminal importin beta binding (sIBB) domain to recruit the receptor importin beta and gain access to the nucleus. In this paper, we demonstrate the sIBB-domain has a bipartite nature, which contains two distinct binding determinants for importin beta. The first determinant spans residues 25-65, and includes the previously identified alphaIBB-region of homology. The second binding determinant encompasses residues 1-24 and resembles region 1011-1035 of the nucleoporin 153 (Nup153). The two binding determinants synergize within the sIBB-domain to confer a low nanomolar binding affinity for importin beta, (Kd ~2nM) in an interaction that, in vitro, is displaced by RanGppNHp. We propose that in vivo the synergy of Nup153 and nuclear RanGTP promotes translocation of U snRNPs into the nucleus.
About this StructureAbout this Structure
2P8Q is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Molecular basis for the recognition of snurportin 1 by importin beta., Mitrousis G, Olia AS, Walker-Kopp N, Cingolani G, J Biol Chem. 2008 Jan 9;. PMID:18187419
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