User:Alice Harmon/Sandbox 4: Difference between revisions
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<Structure load='3UC4' size='400' frame='true' align='left' caption='3uc4 - apo SnRK2.6' scene='55/559985/Aposnrk2_6/3' /> | <Structure load='3UC4' size='400' frame='true' align='left' caption='3uc4 - apo SnRK2.6' scene='55/559985/Aposnrk2_6/3' /> | ||
SNRK2 box is a C-terminal motif that is common to all SNRK2s. It forms a helix that parallels subdomain III. | |||
<scene name='55/559985/Aposnrk2_6critical/1'>Important structures</scene><br> | <scene name='55/559985/Aposnrk2_6critical/1'>Important structures</scene><br> | ||
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<Structure load='3UJG' size='400' frame='true' align='right' caption='3ujg - SnRK2.6 in complex with HAB1' scene='55/559985/Aposnrk2_6/2' /> | <Structure load='3UJG' size='400' frame='true' align='right' caption='3ujg - SnRK2.6 in complex with HAB1' scene='55/559985/Aposnrk2_6/2' /> | ||
The SnRK2.6–HAB1 complex was constructed as a fusion protein with a H6-tag (MAHHHHHHA) at the N-terminus of SnRK2.6 (residues 11–362) fused to HAB1(172–511) with a GSGSAGSAAGS linker. | |||
D296A and E297A surface entropy reduction mutation sites | |||
ABA box | ABA box is acidic sequence in C-terminus that is common to SNRK2s that are regulated by ABA. This sequence binds to PP2C in biochemical assays, but is not seen in the crystal structure. | ||
<scene name='55/559985/Ost1hab1_interaction/1'>interaction zone</scene><br> | <scene name='55/559985/Ost1hab1_interaction/1'>interaction zone</scene><br> | ||
kinase activation loop in blue<br> | kinase activation loop in blue<br> | ||
phosphatase W385 in brown | phosphatase W385 in brown | ||
unused: <scene name='55/559985/Aposnrk2_6/4'>Cysteines in transparent kinase</scene><br> | |||
<scene name='55/559985/Aposnrk2_6/5'>Cysteines in space fill kinase</scene><br> | |||
<scene name='55/559985/Ost1hab1/1'>Cysteines in the complex</scene> | |||
===SNRK2 structures=== | |||
[[3uc3]] ''Arabidopsis thaliana'' SNRK2.3 + Co<sup>2+</sup><br /> | |||
[[3zut]] AtSNRK2.6 (D160A mutant)+ ANP<br /> | |||
[[3zuu]] AtSNRK2.6 (D160A, S175D mutant) + gold<br /> | |||
[[3uc4]] apoAtSNRK2.6 (D59A, E60A mutant)<br /> | |||
[[3udb]] apoAtSNRK2.6 (C131A, C157A, C159A, S7A, s29A, s43A, S166A, T175A) <br /> | |||
'''complex with a protein phosphatase 2C'''<br /> | |||
[[3ujg]] AtSNRK2.6 (D296A) + HAB1 + Mg<sup>2+</sup> | |||
===PP2C structures=== | |||
[[1a6q]] – hPP2C <br /> | |||
[[2iq1]] – hPP2C κ <br /> | |||
[[2p8e]] – hPP2C β<br /> | |||
[[2cm1]] – PP2C + Mn – Micobacterium tuberculosis<br /> | |||
[[3d8k]] – PP2C – Toxoplasma gondii<br /> | |||
[[3jrq]], [[3kdj]], [[3nmn]] – AtPP2C + Pyl1 – Arabidopsis thaliana<br /> | |||
[[3nmt]], [[3kb3]], [[3nmv]], [[3ujl]] – AtPP2C + Pyl2<br /> | |||
[[4ds8]] – AtPP2C + Pyl3 + Mn<br /> | |||
[[3rt0]] – AtPP2C (mutant) + Pyl10<br /> | |||
[[3qn1]], [[3zvu]] – AtPP2C + Pyr1<br /> | |||
[[3ujg]] – AtPP2C + SNRK2<br /> | |||
[[3ujk]] – AtPP2C <br /> | |||
Revision as of 03:35, 25 September 2013
Arabidopsis SnRK2.6 (OST1)Arabidopsis SnRK2.6 (OST1)
|
SNRK2 box is a C-terminal motif that is common to all SNRK2s. It forms a helix that parallels subdomain III.
Activation loop (with gap) in blue
Catalytic loop in orchid
C-helix in yellow
K in chartreuse
Snrk2 box (unique to Snrk's) in turquoise.
C-terminal acidic domain missing.
|
The SnRK2.6–HAB1 complex was constructed as a fusion protein with a H6-tag (MAHHHHHHA) at the N-terminus of SnRK2.6 (residues 11–362) fused to HAB1(172–511) with a GSGSAGSAAGS linker.
D296A and E297A surface entropy reduction mutation sites
ABA box is acidic sequence in C-terminus that is common to SNRK2s that are regulated by ABA. This sequence binds to PP2C in biochemical assays, but is not seen in the crystal structure.
kinase activation loop in blue
phosphatase W385 in brown
unused:
SNRK2 structuresSNRK2 structures
3uc3 Arabidopsis thaliana SNRK2.3 + Co2+
3zut AtSNRK2.6 (D160A mutant)+ ANP
3zuu AtSNRK2.6 (D160A, S175D mutant) + gold
3uc4 apoAtSNRK2.6 (D59A, E60A mutant)
3udb apoAtSNRK2.6 (C131A, C157A, C159A, S7A, s29A, s43A, S166A, T175A)
complex with a protein phosphatase 2C
3ujg AtSNRK2.6 (D296A) + HAB1 + Mg2+
PP2C structuresPP2C structures
1a6q – hPP2C
2iq1 – hPP2C κ
2p8e – hPP2C β
2cm1 – PP2C + Mn – Micobacterium tuberculosis
3d8k – PP2C – Toxoplasma gondii
3jrq, 3kdj, 3nmn – AtPP2C + Pyl1 – Arabidopsis thaliana
3nmt, 3kb3, 3nmv, 3ujl – AtPP2C + Pyl2
4ds8 – AtPP2C + Pyl3 + Mn
3rt0 – AtPP2C (mutant) + Pyl10
3qn1, 3zvu – AtPP2C + Pyr1
3ujg – AtPP2C + SNRK2
3ujk – AtPP2C