2ja7: Difference between revisions

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Revision as of 18:39, 20 March 2008

File:2ja7.jpg

, resolution 3.8Å

Sites:

Ligands:

and

Activity:

DNA-directed RNA polymerase, with EC number 2.7.7.6

Coordinates:

save as pdb, mmCIF, xml

CPD LESION CONTAINING RNA POLYMERASE II ELONGATION COMPLEX C

OverviewOverview

Cells use transcription-coupled repair (TCR) to efficiently eliminate DNA lesions such as ultraviolet light-induced cyclobutane pyrimidine dimers (CPDs). Here we present the structure-based mechanism for the first step in eukaryotic TCR, CPD-induced stalling of RNA polymerase (Pol) II. A CPD in the transcribed strand slowly passes a translocation barrier and enters the polymerase active site. The CPD 5'-thymine then directs uridine misincorporation into messenger RNA, which blocks translocation. Artificial replacement of the uridine by adenosine enables CPD bypass; thus, Pol II stalling requires CPD-directed misincorporation. In the stalled complex, the lesion is inaccessible, and the polymerase conformation is unchanged. This is consistent with nonallosteric recruitment of repair factors and excision of a lesion-containing DNA fragment in the presence of Pol II.

About this StructureAbout this Structure

2JA7 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

CPD damage recognition by transcribing RNA polymerase II., Brueckner F, Hennecke U, Carell T, Cramer P, Science. 2007 Feb 9;315(5813):859-62. PMID:17290000

Page seeded by OCA on Thu Mar 20 17:39:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2ja7.jpg|left|200px]]<br /><applet load="2ja7" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ja7.jpg|left|200px]]
-caption="2ja7, resolution 3.8&Aring;" />
-'''CPD LESION CONTAINING RNA POLYMERASE II ELONGATION COMPLEX C'''<br />
+ {{Structure
+|PDB= 2ja7 |SIZE=350|CAPTION= <scene name='initialview01'>2ja7</scene>, resolution 3.8&Aring;
+|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+M'>AC1</scene>
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]
+|GENE=
+}}
+'''CPD LESION CONTAINING RNA POLYMERASE II ELONGATION COMPLEX C'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-JA7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Known structural/functional Site: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+M'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JA7 OCA].
+JA7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JA7 OCA].
 ==Reference==
-CPD damage recognition by transcribing RNA polymerase II., Brueckner F, Hennecke U, Carell T, Cramer P, Science. 2007 Feb 9;315(5813):859-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17290000 17290000]
+CPD damage recognition by transcribing RNA polymerase II., Brueckner F, Hennecke U, Carell T, Cramer P, Science. 2007 Feb 9;315(5813):859-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17290000 17290000]
 [[Category: DNA-directed RNA polymerase]]
 [[Category: Protein complex]]
@@ Line 48: / Line 57: @@
 [[Category: zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:00:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:39:16 2008''