3vor: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal Structure Analysis of the CofA== | |||
=== | <StructureSection load='3vor' size='340' side='right' caption='[[3vor]], [[Resolution|resolution]] 0.90Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vor]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VOR FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cofA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vor OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vor RCSB], [http://www.ebi.ac.uk/pdbsum/3vor PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CofA, a major pilin subunit of colonization factor antigen III (CFA/III), forms pili that mediate small-intestinal colonization by enterotoxigenic Escherichia coli (ETEC). In this study, the crystal structure of an N-terminally truncated version of CofA was determined by single-wavelength anomalous diffraction (SAD) phasing using five sulfurs in the protein. Given the counterbalance between anomalous signal strength and the undesired X-ray absorption of the solvent, diffraction data were collected at 1.5 A resolution using synchrotron radiation. These data were sufficient to elucidate the sulfur substructure at 1.38 A resolution. The low solvent content (29%) of the crystal necessitated that density modification be performed with an additional 0.9 A resolution data set to reduce the phase error caused by the small sulfur anomalous signal. The CofA structure showed the alphabeta-fold typical of type IVb pilins and showed high structural homology to that of TcpA for toxin-coregulated pili of Vibrio cholerae, including spatial distribution of key residues critical for pilin self-assembly. A pilus-filament model of CofA was built by computational docking and molecular-dynamics simulation using the previously reported filament model of TcpA as a structural template. This model revealed that the CofA filament surface was highly negatively charged and that a 23-residue-long loop between the alpha1 and alpha2 helices filled the gap between the pilin subunits. These characteristics could provide a unique binding epitope for the CFA/III pili of ETEC compared with other type IVb pili. | |||
Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 A resolution by sulfur-SAD phasing.,Fukakusa S, Kawahara K, Nakamura S, Iwashita T, Baba S, Nishimura M, Kobayashi Y, Honda T, Iida T, Taniguchi T, Ohkubo T Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1418-29. Epub 2012 Sep, 13. PMID:22993096<ref>PMID:22993096</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
==See Also== | |||
*[[Pilin|Pilin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Baba, S | [[Category: Baba, S]] | ||
[[Category: Fukakusa, S | [[Category: Fukakusa, S]] | ||
[[Category: Honda, T | [[Category: Honda, T]] | ||
[[Category: Iida, T | [[Category: Iida, T]] | ||
[[Category: Iwasita, T | [[Category: Iwasita, T]] | ||
[[Category: Kawahara, K | [[Category: Kawahara, K]] | ||
[[Category: Kobayashi, Y | [[Category: Kobayashi, Y]] | ||
[[Category: Nakamura, S | [[Category: Nakamura, S]] | ||
[[Category: Nishimura, M | [[Category: Nishimura, M]] | ||
[[Category: Ohkubo, T | [[Category: Ohkubo, T]] | ||
[[Category: Taniguchi, T | [[Category: Taniguchi, T]] | ||
[[Category: Cell adhesion]] | [[Category: Cell adhesion]] | ||
[[Category: Colonization]] | [[Category: Colonization]] | ||
[[Category: Type iv pilin]] | [[Category: Type iv pilin]] | ||