3vkm: Difference between revisions

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-{{STRUCTURE_3vkm|  PDB=3vkm  |  SCENE=  }}
+==Protease-resistant mutant form of Human Galectin-8 in complex with sialyllactose and lactose==
-===Protease-resistant mutant form of Human Galectin-8 in complex with sialyllactose and lactose===
+<StructureSection load='3vkm' size='340' side='right' caption='[[3vkm]], [[Resolution|resolution]] 2.98&Aring;' scene=''>
-{{ABSTRACT_PUBMED_22913484}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3vkm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VKM FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fqz|4fqz]], [[3vkl|3vkl]], [[3vkn|3vkn]], [[3vko|3vko]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LGALS8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vkm RCSB], [http://www.ebi.ac.uk/pdbsum/3vkm PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Galectin-8 is a tandem-repeat-type beta-galactoside-specific animal lectin having N- and C-terminal carbohydrate recognition domains (N-CRD and C-CRD, respectively) with a difference in carbohydrate-binding specificity, involved in cell-matrix interaction, malignant transformation, and cell adhesion. N-CRD exhibits strong affinity for alpha2-3 sialylated oligosaccharides, a feature unique to galectin-8. C-CRD usually exhibits relatively lower affinity for oligosaccharides, but has higher affinity for N-glycan-type branched oligosaccharides than does N-CRD. There have been many structural studies on galectins with a single CRD, but no X-ray structure of a galectin containing both CRDs has been reported. Here, the X-ray structure of a protease-resistant mutant form of human galectin-8 having both CRDs and the novel pseudo-dimer structure of galectin-8 N-CRD in complexes with alpha2-3 sialylated oligosaccharide ligands were determined. The results revealed a difference in specificity between the N- and C-CRDs, and provided new insights into the association of CRDs and/or molecules of galectin-8. (c) 2012 The Authors Journal compilation (c) 2012 FEBS.
-==Function==
+X-ray Structure of a Protease-resistant Mutant Form of Human Galectin-8 with Two Carbohydrate Recognition Domains.,Yoshida H, Yamashita S, Teraoka M, Itoh A, Nakakita SI, Nishi N, Kamitori S FEBS J. 2012 Aug 22. doi: 10.1111/j.1742-4658.2012.08753.x. PMID:22913484<ref>PMID:22913484</ref>
-[[http://www.uniprot.org/uniprot/LEG8_HUMAN LEG8_HUMAN]] Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.<ref>PMID:21288902</ref>
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[3vkm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VKM OCA].
+</div>
-==Reference==
+==See Also==
-<ref group="xtra">PMID:022913484</ref><references group="xtra"/><references/>
+*[[Galectin|Galectin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Kamitori, S.]]
+[[Category: Kamitori, S]]
-[[Category: Nakakita, S.]]
+[[Category: Nakakita, S]]
-[[Category: Nishi, N.]]
+[[Category: Nishi, N]]
-[[Category: Teraoka, M.]]
+[[Category: Teraoka, M]]
-[[Category: Yamashita, S.]]
+[[Category: Yamashita, S]]
-[[Category: Yoshida, H.]]
+[[Category: Yoshida, H]]
 [[Category: Beta-sandwich]]
 [[Category: Carbohydrate binding]]
 [[Category: Oligosaccharide]]
 [[Category: Sugar binding protein]]