4j88: Difference between revisions

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-{{STRUCTURE_4j88|  PDB=4j88  |  SCENE=  }}
+==Dark-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 66==
-===Dark-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 66===
+<StructureSection load='4j88' size='340' side='right' caption='[[4j88]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23620472}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4j88]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J88 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CQ1:[(4Z)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-AZIDOBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CQ1</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j89|4j89]], [[4j8a|4j8a]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j88 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j88 RCSB], [http://www.ebi.ac.uk/pdbsum/4j88 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.
-==Function==
+Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.,Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD Angew Chem Int Ed Engl. 2013 Apr 25. doi: 10.1002/anie.201301490. PMID:23620472<ref>PMID:23620472</ref>
-[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[4j88]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J88 OCA].
+</div>
-==Reference==
+==See Also==
-<ref group="xtra">PMID:023620472</ref><references group="xtra"/><references/>
+*[[Green Fluorescent Protein|Green Fluorescent Protein]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Aequorea victoria]]
-[[Category: Jones, D D.]]
+[[Category: Jones, D D]]
-[[Category: Reddington, S C.]]
+[[Category: Reddington, S C]]
-[[Category: Rizkallah, P J.]]
+[[Category: Rizkallah, P J]]
-[[Category: Tippmann, E M.]]
+[[Category: Tippmann, E M]]
 [[Category: Beta-barrel]]
 [[Category: Chromophore by cyclisation]]