4ggt: Difference between revisions

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-{{STRUCTURE_4ggt|  PDB=4ggt  |  SCENE=  }}
+==Structure of apo Bradavidin2 (Form B)==
-===Structure of apo Bradavidin2 (Form B)===
+<StructureSection load='4ggt' size='340' side='right' caption='[[4ggt]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23661323}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ggt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGT FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y32|2y32]], [[4ggr|4ggr]], [[4ggz|4ggz]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bll1558 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 Bradyrhizobium japonicum USDA 110])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ggt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ggt RCSB], [http://www.ebi.ac.uk/pdbsum/4ggt PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bradavidin II is a biotin-binding protein from Bradyrhizobium japonicum that resembles chicken avidin and bacterial streptavidin. A biophysical characterization was carried out using dynamic light scattering, native mass spectrometry, differential scanning calorimetry, and isothermal titration calorimetry combined with structural characterization using X-ray crystallography. These observations revealed that bradavidin II differs from canonical homotetrameric avidin protein family members in its quaternary structure. In contrast with the other avidins, bradavidin II appears to have a dynamic (transient) oligomeric state in solution. It is monomeric at low protein concentrations but forms higher oligomeric assemblies at higher concentrations. The crystal structure of bradavidin II revealed an important role for Phe42 in shielding the bound ligand from surrounding water molecules, thus functionally replacing the L7,8 loop essential for tight ligand binding in avidin and streptavidin. This bradavidin II characterization opens new avenues for oligomerization-independent biotin-binding protein development.
-==About this Structure==
+The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins.,Leppiniemi J, Meir A, Kahkonen N, Kukkurainen S, Maatta JA, Ojanen M, Janis J, S Kulomaa M, Livnah O, Hytonen VP Protein Sci. 2013 May 10. doi: 10.1002/pro.2281. PMID:23661323<ref>PMID:23661323</ref>
-[[4ggt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGT OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:023661323</ref><references group="xtra"/><references/>
+</div>
+==See Also==
+*[[Avidin|Avidin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bradyrhizobium japonicum usda 110]]
-[[Category: Livnah, O.]]
+[[Category: Livnah, O]]
-[[Category: Meir, A.]]
+[[Category: Meir, A]]
 [[Category: Avidin]]
 [[Category: Beta barrel]]