2hh7: Difference between revisions
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[[Image:2hh7.gif|left|200px]] | [[Image:2hh7.gif|left|200px]] | ||
'''Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.''' | {{Structure | ||
|PDB= 2hh7 |SIZE=350|CAPTION= <scene name='initialview01'>2hh7</scene>, resolution 2.55Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CU1:COPPER (I) ION'>CU1</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2HH7 is a [ | 2HH7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. | ||
==Reference== | ==Reference== | ||
CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator., Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP, Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:[http:// | CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator., Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP, Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17143269 17143269] | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: 4-helix bundle]] | [[Category: 4-helix bundle]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:02 2008'' | ||
Revision as of 18:17, 20 March 2008
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Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.
OverviewOverview
Copper is an essential element that becomes highly cytotoxic when concentrations exceed the capacity of cells to sequester the ion. Here, we identify a new copper-specific repressor (CsoR) of a copper-sensitive operon (cso) in Mycobacterium tuberculosis (Mtb) that is representative of a large, previously uncharacterized family of proteins (DUF156). Electronic and X-ray absorption spectroscopies reveal that CsoR binds a single-monomer mole equivalent of Cu(I) to form a trigonally coordinated (S(2)N) Cu(I) complex. The 2.6-A crystal structure of copper-loaded CsoR shows a homodimeric antiparallel four-helix bundle architecture that represents a novel DNA-binding fold. The Cu(I) is coordinated by Cys36, Cys65' and His61' in a subunit bridging site. Cu(I) binding negatively regulates the binding of CsoR to a DNA fragment encompassing the operator-promoter region of the Mtb cso operon; this results in derepression of the operon in Mtb and the heterologous host Mycobacterium smegmatis. Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed.
About this StructureAbout this Structure
2HH7 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
ReferenceReference
CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator., Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP, Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:17143269
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