2gw4: Difference between revisions
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'''Crystal structure of stony coral fluorescent protein Kaede, red form''' | {{Structure | ||
|PDB= 2gw4 |SIZE=350|CAPTION= <scene name='initialview01'>2gw4</scene>, resolution 1.6Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of stony coral fluorescent protein Kaede, red form''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GW4 is a [ | 2GW4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Trachyphyllia_geoffroyi Trachyphyllia geoffroyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GW4 OCA]. | ||
==Reference== | ==Reference== | ||
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede., Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M, J Mol Biol. 2007 Sep 28;372(4):918-26. Epub 2007 Jun 19. PMID:[http:// | Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede., Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M, J Mol Biol. 2007 Sep 28;372(4):918-26. Epub 2007 Jun 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17692334 17692334] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Trachyphyllia geoffroyi]] | [[Category: Trachyphyllia geoffroyi]] | ||
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[[Category: luminescent protein]] | [[Category: luminescent protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:09:55 2008'' | ||
Revision as of 18:09, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of stony coral fluorescent protein Kaede, red form
OverviewOverview
A coral fluorescent protein from Trachyphyllia geoffroyi, Kaede, possesses a tripeptide of His62-Tyr63-Gly64, which forms a chromophore with green fluorescence. This chromophore's fluorescence turns red following UV light irradiation. We have previously shown that such photoconversion is achieved by a formal beta-elimination reaction, which results in a cleavage of the peptide bond found between the amide nitrogen and the alpha-carbon at His62. However, the stereochemical arrangement of the chromophore and the precise structural basis for this reaction mechanism previously remained unknown. Here, we report the crystal structures of the green and red form of Kaede at 1.4 A and 1.6 A resolutions, respectively. Our structures depict the cleaved peptide bond in the red form. The chromophore conformations both in the green and red forms are similar, except a well-defined water molecule in the proximity of the His62 imidazole ring in the green form. We propose a molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule.
About this StructureAbout this Structure
2GW4 is a Protein complex structure of sequences from Trachyphyllia geoffroyi. Full crystallographic information is available from OCA.
ReferenceReference
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede., Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, Ikura M, J Mol Biol. 2007 Sep 28;372(4):918-26. Epub 2007 Jun 19. PMID:17692334
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