2gtv: Difference between revisions
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'''NMR structure of monomeric chorismate mutase from Methanococcus jannaschii''' | {{Structure | ||
|PDB= 2gtv |SIZE=350|CAPTION= <scene name='initialview01'>2gtv</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID'>TSA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] | |||
|GENE= | |||
}} | |||
'''NMR structure of monomeric chorismate mutase from Methanococcus jannaschii''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GTV is a [ | 2GTV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTV OCA]. | ||
==Reference== | ==Reference== | ||
Structure and dynamics of a molten globular enzyme., Pervushin K, Vamvaca K, Vogeli B, Hilvert D, Nat Struct Mol Biol. 2007 Dec;14(12):1202-6. Epub 2007 Nov 11. PMID:[http:// | Structure and dynamics of a molten globular enzyme., Pervushin K, Vamvaca K, Vogeli B, Hilvert D, Nat Struct Mol Biol. 2007 Dec;14(12):1202-6. Epub 2007 Nov 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17994104 17994104] | ||
[[Category: Chorismate mutase]] | [[Category: Chorismate mutase]] | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
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[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:09:05 2008'' | ||
Revision as of 18:09, 20 March 2008
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| Ligands: | |||||||
| Activity: | Chorismate mutase, with EC number 5.4.99.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of monomeric chorismate mutase from Methanococcus jannaschii
OverviewOverview
Although protein dynamics has been recognized as a potentially important contributor to enzyme catalysis, structural disorder is generally considered to reduce catalytic efficiency. This widely held assumption has recently been challenged by the finding that an engineered chorismate mutase combines high catalytic activity with the properties of a molten globule, a loosely packed and highly dynamic conformational ensemble. Taking advantage of the ordering observed upon ligand binding, we have now used NMR spectroscopy to characterize this enzyme in complex with a transition-state analog. The complex adopts a helix-bundle structure, as designed, but retains unprecedented flexibility on the millisecond timescale across its entire length. Moreover, pre-steady-state kinetics data show that binding occurs by an induced-fit mechanism on the same timescale as the enzymatic reaction, linking global conformational plasticity with efficient catalysis.
About this StructureAbout this Structure
2GTV is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
ReferenceReference
Structure and dynamics of a molten globular enzyme., Pervushin K, Vamvaca K, Vogeli B, Hilvert D, Nat Struct Mol Biol. 2007 Dec;14(12):1202-6. Epub 2007 Nov 11. PMID:17994104
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