2gbl: Difference between revisions
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[[Image:2gbl.gif|left|200px]] | [[Image:2gbl.gif|left|200px]] | ||
'''Crystal Structure of Full Length Circadian Clock Protein KaiC with Phosphorylation Sites''' | {{Structure | ||
|PDB= 2gbl |SIZE=350|CAPTION= <scene name='initialview01'>2gbl</scene>, resolution 2.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] | |||
|GENE= kaiC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1131 Synechococcus sp.]) | |||
}} | |||
'''Crystal Structure of Full Length Circadian Clock Protein KaiC with Phosphorylation Sites''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GBL is a [ | 2GBL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GBL OCA]. | ||
==Reference== | ==Reference== | ||
Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods., Pattanayek R, Williams DR, Pattanayek S, Xu Y, Mori T, Johnson CH, Stewart PL, Egli M, EMBO J. 2006 May 3;25(9):2017-28. Epub 2006 Apr 20. PMID:[http:// | Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods., Pattanayek R, Williams DR, Pattanayek S, Xu Y, Mori T, Johnson CH, Stewart PL, Egli M, EMBO J. 2006 May 3;25(9):2017-28. Epub 2006 Apr 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16628225 16628225] | ||
[[Category: Non-specific serine/threonine protein kinase]] | [[Category: Non-specific serine/threonine protein kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: kaic]] | [[Category: kaic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:02:44 2008'' | ||
Revision as of 18:02, 20 March 2008
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| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | kaiC (Synechococcus sp.) | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Full Length Circadian Clock Protein KaiC with Phosphorylation Sites
OverviewOverview
The cyanobacterial circadian clock can be reconstituted in vitro by mixing recombinant KaiA, KaiB and KaiC proteins with ATP, producing KaiC phosphorylation and dephosphorylation cycles that have a regular rhythm with a ca. 24-h period and are temperature-compensated. KaiA and KaiB are modulators of KaiC phosphorylation, whereby KaiB antagonizes KaiA's action. Here, we present a complete crystallographic model of the Synechococcus elongatus KaiC hexamer that includes previously unresolved portions of the C-terminal regions, and a negative-stain electron microscopy study of S. elongatus and Thermosynechococcus elongatus BP-1 KaiA-KaiC complexes. Site-directed mutagenesis in combination with EM reveals that KaiA binds exclusively to the CII half of the KaiC hexamer. The EM-based model of the KaiA-KaiC complex reveals protein-protein interactions at two sites: the known interaction of the flexible C-terminal KaiC peptide with KaiA, and a second postulated interaction between the apical region of KaiA and the ATP binding cleft on KaiC. This model brings KaiA mutation sites that alter clock period or abolish rhythmicity into contact with KaiC and suggests how KaiA might regulate KaiC phosphorylation.
About this StructureAbout this Structure
2GBL is a Protein complex structure of sequences from Synechococcus sp.. Full crystallographic information is available from OCA.
ReferenceReference
Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods., Pattanayek R, Williams DR, Pattanayek S, Xu Y, Mori T, Johnson CH, Stewart PL, Egli M, EMBO J. 2006 May 3;25(9):2017-28. Epub 2006 Apr 20. PMID:16628225
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