2g6y: Difference between revisions
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[[Image:2g6y.gif|left|200px]] | [[Image:2g6y.gif|left|200px]] | ||
'''Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata''' | {{Structure | ||
|PDB= 2g6y |SIZE=350|CAPTION= <scene name='initialview01'>2g6y</scene>, resolution 1.600Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= ppluGFP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=239963 Pontellina plumata]) | |||
}} | |||
'''Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2G6Y is a [ | 2G6Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pontellina_plumata Pontellina plumata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G6Y OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for the fast maturation of Arthropoda green fluorescent protein., Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM, EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PMID:[http:// | Structural basis for the fast maturation of Arthropoda green fluorescent protein., Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM, EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16936637 16936637] | ||
[[Category: Pontellina plumata]] | [[Category: Pontellina plumata]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rapid maturation]] | [[Category: rapid maturation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:01:09 2008'' | ||
Revision as of 18:01, 20 March 2008
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| , resolution 1.600Å | |||||||
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| Gene: | ppluGFP2 (Pontellina plumata) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata
OverviewOverview
Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.
About this StructureAbout this Structure
2G6Y is a Single protein structure of sequence from Pontellina plumata. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the fast maturation of Arthropoda green fluorescent protein., Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM, EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PMID:16936637
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