2fxl: Difference between revisions
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[[Image:2fxl.gif|left|200px]] | [[Image:2fxl.gif|left|200px]] | ||
'''Urate oxidase from aspergillus flavus complexed with allantoin''' | {{Structure | ||
|PDB= 2fxl |SIZE=350|CAPTION= <scene name='initialview01'>2fxl</scene>, resolution 1.76Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=2AL:1-(2,5-DIOXO-2,5-DIHYDRO-1H-IMIDAZOL-4-YL)UREA'>2AL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] | |||
|GENE= uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 Aspergillus flavus]) | |||
}} | |||
'''Urate oxidase from aspergillus flavus complexed with allantoin''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2FXL is a [ | 2FXL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXL OCA]. | ||
==Reference== | ==Reference== | ||
Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase., Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prange T, FEBS Lett. 2006 Apr 3;580(8):2087-91. Epub 2006 Mar 10. PMID:[http:// | Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase., Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prange T, FEBS Lett. 2006 Apr 3;580(8):2087-91. Epub 2006 Mar 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16545381 16545381] | ||
[[Category: Aspergillus flavus]] | [[Category: Aspergillus flavus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: uric acid degradation]] | [[Category: uric acid degradation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:57:56 2008'' | ||
Revision as of 17:57, 20 March 2008
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| , resolution 1.76Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | uaZ, uox (Aspergillus flavus) | ||||||
| Activity: | Urate oxidase, with EC number 1.7.3.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Urate oxidase from aspergillus flavus complexed with allantoin
OverviewOverview
Urate oxidase from Aspergillus flavus catalyzes the degradation of uric acid to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate. The second degradation step is thought either catalyzed by another specific enzyme, or spontaneous. The structure of the enzyme was known at high resolution by X-ray diffraction of I222 crystals complexed with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the racemic end product of a second degradation step.
About this StructureAbout this Structure
2FXL is a Single protein structure of sequence from Aspergillus flavus. Full crystallographic information is available from OCA.
ReferenceReference
Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase., Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prange T, FEBS Lett. 2006 Apr 3;580(8):2087-91. Epub 2006 Mar 10. PMID:16545381
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