3oq2: Difference between revisions
Jump to navigation
Jump to search
m Protected "3oq2" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of a CRISPR associated protein Cas2 from Desulfovibrio vulgaris== | |||
=== | <StructureSection load='3oq2' size='340' side='right' caption='[[3oq2]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3oq2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OQ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OQ2 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas2, DVUA0135 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=881 Desulfovibrio vulgaris])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oq2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oq2 RCSB], [http://www.ebi.ac.uk/pdbsum/3oq2 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CRISPRs (clustered regularly interspaced short palindromic repeats) provide bacteria and archaea with RNA-guided acquired immunity to invasive DNAs. CRISPR-associated (Cas) proteins carry out the immune effector functions. Cas2 is a universal component of the CRISPR system. Here, a 1.35 A resolution crystal structure of Cas2 from the bacterium Desulfovibrio vulgaris (DvuCas2) is reported. DvuCas2 is a homodimer, with each protomer consisting of an N-terminal betaalphabetabetaalphabeta ferredoxin fold (amino acids 1-78) to which is appended a C-terminal segment (amino acids 79-102) that includes a short 3(10)-helix and a fifth beta-strand. The beta5 strands align with the beta4 strands of the opposite protomers, resulting in two five-stranded antiparallel beta-sheets that form a sandwich at the dimer interface. The DvuCas2 dimer is stabilized by a distinctive network of hydrophilic cross-protomer side-chain interactions. | |||
Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris.,Samai P, Smith P, Shuman S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt, 12):1552-6. Epub 2010 Nov 16. PMID:21139194<ref>PMID:21139194</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Desulfovibrio vulgaris]] | [[Category: Desulfovibrio vulgaris]] | ||
[[Category: Samai, P | [[Category: Samai, P]] | ||
[[Category: Shuman, S | [[Category: Shuman, S]] | ||
[[Category: Smith, P | [[Category: Smith, P]] | ||
[[Category: Crispr]] | [[Category: Crispr]] | ||
[[Category: Ferredoxin fold]] | [[Category: Ferredoxin fold]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
Revision as of 10:16, 19 December 2014
Structure of a CRISPR associated protein Cas2 from Desulfovibrio vulgarisStructure of a CRISPR associated protein Cas2 from Desulfovibrio vulgaris
Structural highlights
Publication Abstract from PubMedCRISPRs (clustered regularly interspaced short palindromic repeats) provide bacteria and archaea with RNA-guided acquired immunity to invasive DNAs. CRISPR-associated (Cas) proteins carry out the immune effector functions. Cas2 is a universal component of the CRISPR system. Here, a 1.35 A resolution crystal structure of Cas2 from the bacterium Desulfovibrio vulgaris (DvuCas2) is reported. DvuCas2 is a homodimer, with each protomer consisting of an N-terminal betaalphabetabetaalphabeta ferredoxin fold (amino acids 1-78) to which is appended a C-terminal segment (amino acids 79-102) that includes a short 3(10)-helix and a fifth beta-strand. The beta5 strands align with the beta4 strands of the opposite protomers, resulting in two five-stranded antiparallel beta-sheets that form a sandwich at the dimer interface. The DvuCas2 dimer is stabilized by a distinctive network of hydrophilic cross-protomer side-chain interactions. Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris.,Samai P, Smith P, Shuman S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt, 12):1552-6. Epub 2010 Nov 16. PMID:21139194[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||