2fmx: Difference between revisions
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'''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)''' | {{Structure | ||
|PDB= 2fmx |SIZE=350|CAPTION= <scene name='initialview01'>2fmx</scene>, resolution 1.82Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2FMX is a [ | 2FMX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMX OCA]. | ||
==Reference== | ==Reference== | ||
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:[http:// | An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16899220 16899220] | ||
[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sar1]] | [[Category: sar1]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:54:22 2008'' | ||
Revision as of 17:54, 20 March 2008
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| , resolution 1.82Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)
OverviewOverview
Mg2+ is essential for guanosine triphosphatase activity and plays key roles in guanine nucleotide binding and preserving the structural integrity of GTP-binding proteins. To understand the structural basis for Mg2+ function during the GDP/GTP exchange process, we determined the crystal structure of Delta9-Sar1-GDP at low Mg2+ concentration at 1.8A. Two Sar1-GDP molecules in the crystal form a dimer with Mg2+ presenting only in molecule B but not in molecule A. The absence of Mg2+ induces significant conformational changes in the switch I region in molecule A that shows similarities with those of Ha-Ras bound to Sos. The current structure reveals an important regulatory role for Mg2+. We suggest that guanine nucleotide exchange factor may utilize this feature to generate an open conformation for GDP/GTP exchange. Furthermore, we propose a mechanism for COPII assembly and disassembly in which dimerization of Sar1 plays an important role.
About this StructureAbout this Structure
2FMX is a Single protein structure of sequence from Cricetulus griseus. Full crystallographic information is available from OCA.
ReferenceReference
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:16899220
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