2fmd: Difference between revisions

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File:2fmd.gif

, resolution 1.9Å

Ligands:

and

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Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin

OverviewOverview

The crystal structure of the seed lectin from the tropical legume Bowringia milbraedii was determined in complex with the disaccharide ligand Man(alpha1-2)Man. In solution, the protein exhibits a dynamic dimer-tetramer equilibrium, consistent with the concanavalin A-type tetramer observed in the crystal. Contacts between the tetramers are mediated almost exclusively through the carbohydrate ligand, resulting in a crystal lattice virtually identical to that of the concanavalin-A:Man(alpha1-2)Man complex, even though both proteins have less than 50% sequence identity. The disaccharide binds exclusively in a "downstream" binding mode, with the non-reducing mannose occupying the monosaccharide-binding site. The reducing mannose is bound in a predominantly polar subsite involving Tyr131, Gln218, and Tyr219.

About this StructureAbout this Structure

2FMD is a Single protein structure of sequence from Bowringia mildbraedii. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of carbohydrate recognition by a Man(alpha1-2)Man-specific lectin from Bowringia milbraedii., Buts L, Garcia-Pino A, Wyns L, Loris R, Glycobiology. 2006 Jul;16(7):635-40. Epub 2006 Mar 27. PMID:16567368

Page seeded by OCA on Thu Mar 20 16:54:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2fmd.gif|left|200px]]<br /><applet load="2fmd" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fmd.gif|left|200px]]
-caption="2fmd, resolution 1.9&Aring;" />
-'''Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin'''<br />
+ {{Structure
+|PDB= 2fmd |SIZE=350|CAPTION= <scene name='initialview01'>2fmd</scene>, resolution 1.9&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-FMD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bowringia_mildbraedii Bowringia mildbraedii] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMD OCA].
+FMD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bowringia_mildbraedii Bowringia mildbraedii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMD OCA].
 ==Reference==
-Structural basis of carbohydrate recognition by a Man(alpha1-2)Man-specific lectin from Bowringia milbraedii., Buts L, Garcia-Pino A, Wyns L, Loris R, Glycobiology. 2006 Jul;16(7):635-40. Epub 2006 Mar 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16567368 16567368]
+Structural basis of carbohydrate recognition by a Man(alpha1-2)Man-specific lectin from Bowringia milbraedii., Buts L, Garcia-Pino A, Wyns L, Loris R, Glycobiology. 2006 Jul;16(7):635-40. Epub 2006 Mar 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16567368 16567368]
 [[Category: Bowringia mildbraedii]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: protein-carbohydrate complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:22:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:54:13 2008''