2fhz: Difference between revisions
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[[Image:2fhz.gif|left|200px]] | [[Image:2fhz.gif|left|200px]] | ||
'''Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein''' | {{Structure | ||
|PDB= 2fhz |SIZE=350|CAPTION= <scene name='initialview01'>2fhz</scene>, resolution 1.15Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= imm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), col ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2FHZ is a [ | 2FHZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHZ OCA]. | ||
==Reference== | ==Reference== | ||
Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity protein., Luna-Chavez C, Lin YL, Huang RH, J Mol Biol. 2006 Apr 28;358(2):571-9. Epub 2006 Feb 24. PMID:[http:// | Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity protein., Luna-Chavez C, Lin YL, Huang RH, J Mol Biol. 2006 Apr 28;358(2):571-9. Epub 2006 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16524591 16524591] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:42 2008'' | ||
Revision as of 17:52, 20 March 2008
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| , resolution 1.15Å | |||||||
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| Gene: | imm (Escherichia coli), col (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein
OverviewOverview
Colicin E5 is a tRNA-specific ribonuclease that recognizes and cleaves four tRNAs in Escherichia coli that contain the hypermodified nucleoside queuosine (Q) at the wobble position. Cells that produce colicin E5 also synthesize the cognate immunity protein (Im5) that rapidly and tightly associates with colicin E5 to prevent it from cleaving its own tRNAs to avoid suicide. We report here the crystal structure of Im5 in a complex with the activity domain of colicin E5 (E5-CRD) at 1.15A resolution. The structure reveals an extruded domain from Im5 that docks into the recessed RNA binding cleft in E5-CRD, resulting in extensive interactions between the two proteins. The interactions are primarily hydrophilic, with an interface that contains complementary surface charges between the two proteins. Detailed interactions in three separate regions of the interface account for specific recognition of colicin E5 by Im5. Furthermore, single-site mutational studies of Im5 confirmed the important role of particular residues in recognition and binding of colicin E5. Structural comparison of the complex reported here with E5-CRD alone, as well as with a docking model of RNA-E5-CRD, indicates that Im5 achieves its inhibition by physically blocking the cleft in colicin E5 that engages the RNA substrate.
About this StructureAbout this Structure
2FHZ is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity protein., Luna-Chavez C, Lin YL, Huang RH, J Mol Biol. 2006 Apr 28;358(2):571-9. Epub 2006 Feb 24. PMID:16524591
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