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{{STRUCTURE_3nmd|  PDB=3nmd  |  SCENE=  }}
==Crystal structure of the leucine zipper domain of cGMP dependent protein kinase I beta==
===Crystal structure of the leucine zipper domain of cGMP dependent protein kinase I beta===
<StructureSection load='3nmd' size='340' side='right' caption='[[3nmd]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
{{ABSTRACT_PUBMED_20826808}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3nmd]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NMD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRKG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nmd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nmd RCSB], [http://www.ebi.ac.uk/pdbsum/3nmd PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cyclic GMP-dependent protein kinase (PKG) is a key mediator of the nitric oxide/cGMP signaling pathway and plays a central role in regulating cardiovascular and neuronal functions. The N-terminal approximately 50 amino acids of the kinase are required for homodimerization and association with isoform-specific PKG-anchoring proteins (GKAPs), which target the kinase to specific substrates. To understand the molecular details of PKG dimerization and gain insight into its association with GKAPs, we solved a crystal structure of the PKG Ibeta dimerization/docking domain. Our structure provides molecular details of this unique leucine/isoleucine zipper, revealing specific hydrophobic and ionic interactions that mediate dimerization and demonstrating the topology of the GKAP interaction surface.


==Function==
A crystal structure of the cyclic GMP-dependent protein kinase I{beta} dimerization/docking domain reveals molecular details of isoform-specific anchoring.,Casteel DE, Smith-Nguyen EV, Sankaran B, Roh SH, Pilz RB, Kim C J Biol Chem. 2010 Oct 22;285(43):32684-8. Epub 2010 Sep 8. PMID:20826808<ref>PMID:20826808</ref>
[[http://www.uniprot.org/uniprot/Q6P5T7_HUMAN Q6P5T7_HUMAN]] Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.<ref>PMID:8182057</ref> <ref>PMID:10567269</ref> <ref>PMID:11162591</ref> <ref>PMID:12082086</ref> <ref>PMID:11723116</ref> <ref>PMID:14608379</ref> <ref>PMID:15194681</ref> <ref>PMID:16990611</ref> <ref>PMID:21402151</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[3nmd]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMD OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020826808</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: BSGC, Berkeley Structural Genomics Center.]]
[[Category: Structural genomic]]
[[Category: Casteel, D E.]]
[[Category: Casteel, D E]]
[[Category: Kim, C.]]
[[Category: Kim, C]]
[[Category: Sankaran, B.]]
[[Category: Sankaran, B]]
[[Category: Smith-Nguyen, E V.]]
[[Category: Smith-Nguyen, E V]]
[[Category: Berkeley structural genomics center]]
[[Category: Bsgc]]
[[Category: Bsgc]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
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[[Category: Inositol triphosphate receptor-associated pkg substrate]]
[[Category: Inositol triphosphate receptor-associated pkg substrate]]
[[Category: Leucine zipper]]
[[Category: Leucine zipper]]
[[Category: Structural genomic]]
[[Category: Transcriptional regulator tfii-i]]
[[Category: Transcriptional regulator tfii-i]]
[[Category: Transferase]]
[[Category: Transferase]]

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