2erb: Difference between revisions
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[[Image:2erb.gif|left|200px]] | [[Image:2erb.gif|left|200px]] | ||
'''AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG''' | {{Structure | ||
|PDB= 2erb |SIZE=350|CAPTION= <scene name='initialview01'>2erb</scene>, resolution 1.50Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=PEU:2,5,8,11,14,17,20,23,26,29,32,35,38,41,44,47,50,53,56,59,62,65,68,71,74,77,80-HEPTACOSAOXADOOCTACONTAN-82-OL'>PEU</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2ERB is a [ | 2ERB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERB OCA]. | ||
==Reference== | ==Reference== | ||
The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:[http:// | The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16300742 16300742] | ||
[[Category: Anopheles gambiae]] | [[Category: Anopheles gambiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: MG]] | [[Category: MG]] | ||
[[Category: PEU]] | [[Category: PEU]] | ||
[[Category: | [[Category: disulfide]] | ||
[[Category: helix]] | [[Category: helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:43:29 2008'' | ||
Revision as of 17:43, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG
OverviewOverview
The Anopheles gambiae mosquito is the main vector of malaria transmission in sub-Saharan Africa. We present here a 1.5A crystal structure of AgamOBP1, an odorant binding protein (OBP) from the A. gambiae mosquito. The protein crystallized as a dimer with a unique binding pocket consisting of a continuous tunnel running through both subunits of the dimer and occupied by a PEG molecule. We demonstrate that AgamOBP1 undergoes a pH dependent conformational change that is associated with reduced ligand binding. A predominance of acid-labile hydrogen bonds involving the C-terminal loop suggests a mechanism in which a drop in pH causes C-terminal loop to open, leaving the binding tunnel solvent exposed, thereby lowering binding affinity for ligand. Because proteins from two distantly related insects also undergo a pH dependent conformational change involving the C-terminus that is associated with reduced ligand affinity, our results suggest a common mechanism for OBP activity.
About this StructureAbout this Structure
2ERB is a Single protein structure of sequence from Anopheles gambiae. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:16300742
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