2dqs: Difference between revisions

← Older edit Newer edit →

Revision as of 17:30, 20 March 2008

File:2dqs.jpg

, resolution 2.50Å

Ligands:

, , , and

Activity:

Calcium-transporting ATPase, with EC number 3.6.3.8

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of the calcium pump with amppcp in the absence of calcium

OverviewOverview

Calcium ATPase is a member of the P-type ATPases that transport ions across the membrane against a concentration gradient. Here we have solved the crystal structure of the calcium ATPase of skeletal muscle sarcoplasmic reticulum (SERCA1a) at 2.6 A resolution with two calcium ions bound in the transmembrane domain, which comprises ten alpha-helices. The two calcium ions are located side by side and are surrounded by four transmembrane helices, two of which are unwound for efficient coordination geometry. The cytoplasmic region consists of three well separated domains, with the phosphorylation site in the central catalytic domain and the adenosine-binding site on another domain. The phosphorylation domain has the same fold as haloacid dehalogenase. Comparison with a low-resolution electron density map of the enzyme in the absence of calcium and with biochemical data suggests that large domain movements take place during active transport.

About this StructureAbout this Structure

2DQS is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution., Toyoshima C, Nakasako M, Nomura H, Ogawa H, Nature. 2000 Jun 8;405(6787):647-55. PMID:10864315

Page seeded by OCA on Thu Mar 20 16:30:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2dqs.jpg|left|200px]]<br /><applet load="2dqs" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2dqs.jpg|left|200px]]
-caption="2dqs, resolution 2.50&Aring;" />
-'''Crystal structure of the calcium pump with amppcp in the absence of calcium'''<br />
+ {{Structure
+|PDB= 2dqs |SIZE=350|CAPTION= <scene name='initialview01'>2dqs</scene>, resolution 2.50&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=TG1:OCTANOIC+ACID+[3S-[3ALPHA,+3ABETA,+4ALPHA,+6BETA,+6ABETA,+7BETA,+8ALPHA(Z),+9BALPHA]]-6-(ACETYLOXY)-2,3,-3A,4,5,6,6A,7,8,9B-DECAHYDRO-3,3A-DIHYDROXY-3,6,9-TRIMETHYL-8-[(2-METHYL-1-OXO-2-BUTENYL)OXY]-2-OXO-4-(1-OXOBUTOXY)-AZULENO[4,5-B]FURAN-7-YL+ESTER'>TG1</scene> and <scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8]
+|GENE=
+}}
+'''Crystal structure of the calcium pump with amppcp in the absence of calcium'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DQS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=ACP:'>ACP</scene>, <scene name='pdbligand=TG1:'>TG1</scene> and <scene name='pdbligand=PTY:'>PTY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DQS OCA].
+DQS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DQS OCA].
 ==Reference==
-Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution., Toyoshima C, Nakasako M, Nomura H, Ogawa H, Nature. 2000 Jun 8;405(6787):647-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10864315 10864315]
+Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution., Toyoshima C, Nakasako M, Nomura H, Ogawa H, Nature. 2000 Jun 8;405(6787):647-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864315 10864315]
 [[Category: Calcium-transporting ATPase]]
 [[Category: Oryctolagus cuniculus]]
@@ Line 26: / Line 35: @@
 [[Category: p-type atpase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:01:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:30:22 2008''