2dea: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2dea.gif|left|200px]] | [[Image:2dea.gif|left|200px]] | ||
'''Crystal Structure of the Aminopeptidase of Aeromonas proteolytica at pH 4.7''' | {{Structure | ||
|PDB= 2dea |SIZE=350|CAPTION= <scene name='initialview01'>2dea</scene>, resolution 1.24Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Bacterial_leucyl_aminopeptidase Bacterial leucyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.10 3.4.11.10] | |||
|GENE= | |||
}} | |||
'''Crystal Structure of the Aminopeptidase of Aeromonas proteolytica at pH 4.7''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2DEA is a [ | 2DEA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEA OCA]. | ||
==Reference== | ==Reference== | ||
The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica., Desmarais W, Bienvenue DL, Bzymek KP, Petsko GA, Ringe D, Holz RC, J Biol Inorg Chem. 2006 Jun;11(4):398-408. Epub 2006 Apr 5. PMID:[http:// | The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica., Desmarais W, Bienvenue DL, Bzymek KP, Petsko GA, Ringe D, Holz RC, J Biol Inorg Chem. 2006 Jun;11(4):398-408. Epub 2006 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16596389 16596389] | ||
[[Category: Bacterial leucyl aminopeptidase]] | [[Category: Bacterial leucyl aminopeptidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: zinc enzyme]] | [[Category: zinc enzyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:26:14 2008'' | ||
Revision as of 17:26, 20 March 2008
| |||||||
| , resolution 1.24Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Bacterial leucyl aminopeptidase, with EC number 3.4.11.10 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Aminopeptidase of Aeromonas proteolytica at pH 4.7
OverviewOverview
The aminopeptidase from Aeromonas proteolytica (AAP) contains two zinc ions in the active site and catalyzes the degradation of peptides. Herein we report the crystal structures of AAP at 0.95-A resolution at neutral pH and at 1.24-A resolution at low pH. The combination of these structures allowed the precise modeling of atomic positions, the identification of the metal bridging oxygen species, and insight into the physical properties of the metal ions. On the basis of these structures, a new putative catalytic mechanism is proposed for AAP that is likely relevant to all binuclear metalloproteases.
About this StructureAbout this Structure
2DEA is a Single protein structure of sequence from Vibrio proteolyticus. Full crystallographic information is available from OCA.
ReferenceReference
The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica., Desmarais W, Bienvenue DL, Bzymek KP, Petsko GA, Ringe D, Holz RC, J Biol Inorg Chem. 2006 Jun;11(4):398-408. Epub 2006 Apr 5. PMID:16596389
Page seeded by OCA on Thu Mar 20 16:26:14 2008