1of5: Difference between revisions
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==Overview== | ==Overview== | ||
The association between Mtr2 and Mex67 is essential for the nuclear export, of bulk messenger RNA in yeast. In metazoans, the analogous function is, carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly, conserved proteins, their binding partners, Mtr2 and p15, share no, sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with, the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like, family and interacts with Mex67, forming a complex with a similar, structural architecture to that of TAP-p15. Mtr2 fulfils an analogous, function to that of human p15 in maintaining the structural integrity of, the heterodimer. In addition, Mtr2 presents a long internal loop, which, . | The association between Mtr2 and Mex67 is essential for the nuclear export, of bulk messenger RNA in yeast. In metazoans, the analogous function is, carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly, conserved proteins, their binding partners, Mtr2 and p15, share no, sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with, the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like, family and interacts with Mex67, forming a complex with a similar, structural architecture to that of TAP-p15. Mtr2 fulfils an analogous, function to that of human p15 in maintaining the structural integrity of, the heterodimer. In addition, Mtr2 presents a long internal loop, which, contains residues that affect the export of the large ribosomal subunit. | ||
==About this Structure== | ==About this Structure== | ||
1OF5 is a | 1OF5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OF5 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:18:34 2007'' | ||
Revision as of 15:13, 5 November 2007
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CRYSTAL STRUCTURE OF MEX67-MTR2
OverviewOverview
The association between Mtr2 and Mex67 is essential for the nuclear export, of bulk messenger RNA in yeast. In metazoans, the analogous function is, carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly, conserved proteins, their binding partners, Mtr2 and p15, share no, sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with, the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like, family and interacts with Mex67, forming a complex with a similar, structural architecture to that of TAP-p15. Mtr2 fulfils an analogous, function to that of human p15 in maintaining the structural integrity of, the heterodimer. In addition, Mtr2 presents a long internal loop, which, contains residues that affect the export of the large ribosomal subunit.
About this StructureAbout this Structure
1OF5 is a Protein complex structure of sequences from Saccharomyces cerevisiae with HG as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15., Fribourg S, Conti E, EMBO Rep. 2003 Jul;4(7):699-703. PMID:12835756
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