2c39: Difference between revisions

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RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP

OverviewOverview

The exosome is a macromolecular complex that plays fundamental roles in the biogenesis and turnover of a large number of RNA species. Here we report the crystal structures of the Rrp41-Rrp42 core complex of the S. solfataricus exosome bound to short single-stranded RNAs and to ADP. The RNA binding cleft recognizes four nucleotides in a sequence-unspecific manner, mainly by electrostatic interactions with the phosphate groups. Interactions at the 2' hydroxyls of the sugars provide specificity for RNA over DNA. The structures show both the bound substrate and the cleaved product of the reaction, suggesting a catalytic mechanism for the 3'-5' phosphorolytic activity of the exosome.

About this StructureAbout this Structure

2C39 is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928

Page seeded by OCA on Thu Mar 20 16:10:16 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:2c39.gif|left|200px]]<br /><applet load="2c39" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2c39.gif|left|200px]]
-caption="2c39, resolution 3.30&Aring;" />
-'''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP'''<br />
+ {{Structure
+|PDB= 2c39 |SIZE=350|CAPTION= <scene name='initialview01'>2c39</scene>, resolution 3.30&Aring;
+|SITE= <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+X'>AC1</scene>
+|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH ADP'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-C39 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C39 OCA].
+C39 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C39 OCA].
 ==Reference==
-Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16285928 16285928]
+Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16285928 16285928]
 [[Category: Protein complex]]
 [[Category: Sulfolobus solfataricus]]
@@ Line 28: / Line 37: @@
 [[Category: rrp42]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:44:23 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:10:16 2008''