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==The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.== | |||
<StructureSection load='4a1r' size='340' side='right' caption='[[4a1r]], [[Resolution|resolution]] 1.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4a1r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A1R FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a1r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a1r RCSB], [http://www.ebi.ac.uk/pdbsum/4a1r PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lip is a membrane-bound lipoprotein and a core component of the type VI secretion system found in Gram-negative bacteria. The structure of a Lip construct (residues 29-176) from Serratia marcescens (SmLip) has been determined at 1.92 A resolution. Experimental phases were derived using a single-wavelength anomalous dispersion approach on a sample cocrystallized with iodide. The membrane localization of the native protein was confirmed. The structure is that of the globular domain lacking only the lipoprotein signal peptide and the lipidated N-terminus of the mature protein. The protein fold is dominated by an eight-stranded beta-sandwich and identifies SmLip as a new member of the transthyretin family of proteins. Transthyretin and the only other member of the family fold, 5-hydroxyisourate hydrolase, form homotetramers important for their function. The asymmetric unit of SmLip is a tetramer with 222 symmetry, but the assembly is distinct from that previously noted for the transthyretin protein family. However, structural comparisons and bacterial two-hybrid data suggest that the SmLip tetramer is not relevant to its role as a core component of the type VI secretion system, but rather reflects a propensity for SmLip to participate in protein-protein interactions. A relatively low level of sequence conservation amongst Lip homologues is noted and is restricted to parts of the structure that might be involved in interactions with physiological partners. | |||
The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system.,Rao VA, Shepherd SM, English G, Coulthurst SJ, Hunter WN Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1065-72. Epub 2011 Nov, 18. PMID:22120744<ref>PMID:22120744</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
< | </div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Coulthurst, S J | [[Category: Coulthurst, S J]] | ||
[[Category: English, G | [[Category: English, G]] | ||
[[Category: Hunter, W N | [[Category: Hunter, W N]] | ||
[[Category: Rao, V A | [[Category: Rao, V A]] | ||
[[Category: Shepherd, S M | [[Category: Shepherd, S M]] | ||
[[Category: Beta-sandwich]] | [[Category: Beta-sandwich]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
[[Category: T6ss]] | [[Category: T6ss]] | ||