2bng: Difference between revisions

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Revision as of 17:04, 20 March 2008

File:2bng.gif

, resolution 2.50Å

Sites:

Ligands:

Activity:

Limonene-1,2-epoxide hydrolase, with EC number 3.3.2.8

Coordinates:

save as pdb, mmCIF, xml

STRUCTURE OF AN M.TUBERCULOSIS LEH-LIKE EPOXIDE HYDROLASE

OverviewOverview

Epoxide hydrolases are vital to many organisms by virtue of their roles in detoxification, metabolism and processing of signaling molecules. The Mycobacterium tuberculosis genome encodes an unusually large number of epoxide hydrolases, suggesting that they might be of particular importance to these bacteria. We report here the first structure of an epoxide hydrolase from M.tuberculosis, solved to a resolution of 2.5 A using single-wavelength anomalous dispersion (SAD) from a selenomethionine-substituted protein. The enzyme features a deep active-site pocket created by the packing of three helices onto a curved six-stranded beta-sheet. This structure is similar to a previously described limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis and unlike the alpha/beta-hydrolase fold typical of mammalian epoxide hydrolases (EH). A number of changes in the mycobacterial enzyme create a wider and deeper substrate-binding pocket than is found in its Rhodococcus homologue. Interestingly, each structure contains a different type of endogenous ligand of unknown origin bound in its active site. As a consequence of its wider substrate-binding pocket, the mycobacterial EH is capable of hydrolyzing long or bulky lipophilic epoxides such as 10,11-epoxystearic acid and cholesterol 5,6-oxide at appreciable rates, suggesting that similar compound(s) will serve as its physiological substrate(s).

About this StructureAbout this Structure

2BNG is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of an atypical epoxide hydrolase from Mycobacterium tuberculosis gives insights into its function., Johansson P, Unge T, Cronin A, Arand M, Bergfors T, Jones TA, Mowbray SL, J Mol Biol. 2005 Sep 2;351(5):1048-56. PMID:16051262

Page seeded by OCA on Thu Mar 20 16:04:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2bng.gif|left|200px]]<br /><applet load="2bng" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bng.gif|left|200px]]
-caption="2bng, resolution 2.50&Aring;" />
-'''STRUCTURE OF AN M.TUBERCULOSIS LEH-LIKE EPOXIDE HYDROLASE'''<br />
+ {{Structure
+|PDB= 2bng |SIZE=350|CAPTION= <scene name='initialview01'>2bng</scene>, resolution 2.50&Aring;
+|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+B'>AC1</scene>
+|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8]
+|GENE=
+}}
+'''STRUCTURE OF AN M.TUBERCULOSIS LEH-LIKE EPOXIDE HYDROLASE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-BNG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] Known structural/functional Site: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNG OCA].
+BNG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNG OCA].
 ==Reference==
-Structure of an atypical epoxide hydrolase from Mycobacterium tuberculosis gives insights into its function., Johansson P, Unge T, Cronin A, Arand M, Bergfors T, Jones TA, Mowbray SL, J Mol Biol. 2005 Sep 2;351(5):1048-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16051262 16051262]
+Structure of an atypical epoxide hydrolase from Mycobacterium tuberculosis gives insights into its function., Johansson P, Unge T, Cronin A, Arand M, Bergfors T, Jones TA, Mowbray SL, J Mol Biol. 2005 Sep 2;351(5):1048-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16051262 16051262]
 [[Category: Limonene-1,2-epoxide hydrolase]]
 [[Category: Mycobacterium tuberculosis]]
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 [[Category: m tuberculosis]]
 [[Category: spine]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: structural proteomics in europe]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:04:15 2008''