Molecular Playground/CheA: Difference between revisions
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In Progress: One of the [http://www.proteopedia.org/wiki/index.php/CBI_Molecules CBI Molecules] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program at UMass Amherst]. | In Progress: One of the [http://www.proteopedia.org/wiki/index.php/CBI_Molecules CBI Molecules] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program at UMass Amherst]. | ||
<Structure load='1b3q' size='500' frame='true' align='right' caption='Structure of histidine kinase CheA, [[1b3q]]' scene='User:Elizabeth_R._Haglin/Sandbox_1/P3p4p5/3'> | <Structure load='1b3q' size='500' frame='true' align='right' caption='Structure of histidine kinase CheA, [[1b3q]]' scene='User:Elizabeth_R._Haglin/Sandbox_1/P3p4p5/3'> | ||
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== Biological context == | == Biological context == | ||
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== Structure == | == Structure == | ||
'''CheA''' exists as a homodimer of 71-kDa subunits. Each monomer catalyzes an ATP-dependent ''trans''-phosphorylation of a histidine. A monomer contains five domains (P1-P5 from N- to C-terminus) connected by highly dynamic linkers of various lengths. Each domain has a distinct function.Due to the size of CheA, the solved structures available to date do not have all five domains. The PDB files included in this proteopedia page are <u><scene name='User:Elizabeth_R._Haglin/Sandbox_1/P3p4p5/3'>1B3Q</scene></u> with a dimer of <b><font color='deepskyblue'>P3</font><font color='limegreen'>P4</font><font color='mediumorchid'>P5</font></b> and <u><scene name='User:Elizabeth_R._Haglin/Sandbox_1/P1_p2_chey/2'>2LP4</scene></u> which contains a <b><font color='gold'>P1</font><font color='hotpink'>P2</font>-<font color='red'>CheY</font></b> complex. | '''CheA''' exists as a homodimer of 71-kDa subunits. Each monomer catalyzes an ATP-dependent ''trans''-phosphorylation of a histidine. A monomer contains five domains (P1-P5 from N- to C-terminus) connected by highly dynamic linkers of various lengths. Each domain has a distinct function.Due to the size of CheA, the solved structures available to date do not have all five domains. The PDB files included in this proteopedia page are <u><scene name='User:Elizabeth_R._Haglin/Sandbox_1/P3p4p5/3'>1B3Q</scene></u> with a dimer of <b><font color='deepskyblue'>P3</font><font color='limegreen'>P4</font><font color='mediumorchid'>P5</font></b> and <u><scene name='User:Elizabeth_R._Haglin/Sandbox_1/P1_p2_chey/2'>2LP4</scene></u> which contains a <b><font color='gold'>P1</font><font color='hotpink'>P2</font>-<font color='red'>CheY</font></b> complex. | ||
*<b><font color='gold'>P1</font></b>: histidine phosphotransfer domain (HPt) mediates phosphate transfer from ATP to CheY | *<b><font color='gold'>P1</font></b>: histidine phosphotransfer domain (HPt) mediates phosphate transfer from ATP to CheY | ||