2bku: Difference between revisions
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[[Image:2bku.gif|left|200px]] | [[Image:2bku.gif|left|200px]] | ||
'''KAP95P:RANGTP COMPLEX''' | {{Structure | ||
|PDB= 2bku |SIZE=350|CAPTION= <scene name='initialview01'>2bku</scene>, resolution 2.70Å | |||
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''KAP95P:RANGTP COMPLEX''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2BKU is a [ | 2BKU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 2BKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKU OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for nuclear import complex dissociation by RanGTP., Lee SJ, Matsuura Y, Liu SM, Stewart M, Nature. 2005 Jun 2;435(7042):693-6. Epub 2005 May 1. PMID:[http:// | Structural basis for nuclear import complex dissociation by RanGTP., Lee SJ, Matsuura Y, Liu SM, Stewart M, Nature. 2005 Jun 2;435(7042):693-6. Epub 2005 May 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15864302 15864302] | ||
[[Category: Canis lupus familiaris]] | [[Category: Canis lupus familiaris]] | ||
[[Category: Importins]] | [[Category: Importins]] | ||
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[[Category: rangtp]] | [[Category: rangtp]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:03:16 2008'' | ||
Revision as of 17:03, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
KAP95P:RANGTP COMPLEX
OverviewOverview
Nuclear protein import is mediated mainly by the transport factor importin-beta that binds cytoplasmic cargo, most often via the importin-alpha adaptor, and then transports it through nuclear pore complexes. This active transport is driven by disassembly of the import complex by nuclear RanGTP. The switch I and II loops of Ran change conformation with nucleotide state, and regulate its interactions with nuclear trafficking components. Importin-beta consists of 19 HEAT repeats that are based on a pair of antiparallel alpha-helices (referred to as the A- and B-helices). The HEAT repeats stack to yield two C-shaped arches, linked together to form a helicoidal molecule that has considerable conformational flexibility. Here we present the structure of full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides a basis for understanding the crucial cargo-release step of nuclear import. We identify a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p. This interaction produces a change in helicoidal pitch that locks Kap95p in a conformation that cannot bind importin-alpha or cargo. We suggest an allosteric mechanism for nuclear import complex disassembly by RanGTP.
About this StructureAbout this Structure
2BKU is a Protein complex structure of sequences from Canis lupus familiaris and Saccharomyces cerevisiae. The following page contains interesting information on the relation of 2BKU with [Importins]. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for nuclear import complex dissociation by RanGTP., Lee SJ, Matsuura Y, Liu SM, Stewart M, Nature. 2005 Jun 2;435(7042):693-6. Epub 2005 May 1. PMID:15864302
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