3ova: Difference between revisions

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-{{STRUCTURE_3ova|  PDB=3ova  |  SCENE=  }}
+==How the CCA-adding Enzyme Selects Adenine over Cytosine in Position 76 of tRNA==
-===How the CCA-adding Enzyme Selects Adenine over Cytosine in Position 76 of tRNA===
+<StructureSection load='3ova' size='340' side='right' caption='[[3ova]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
-{{ABSTRACT_PUBMED_21071662}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3ova]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OVA FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ouy|3ouy]], [[3ov7|3ov7]], [[3ovb|3ovb]], [[3ovs|3ovs]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ova FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ova OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ova RCSB], [http://www.ebi.ac.uk/pdbsum/3ova PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+CCA-adding enzymes [ATP(CTP):tRNA nucleotidyltransferases] add CCA onto the 3' end of transfer RNA (tRNA) precursors without using a nucleic acid template. Although the mechanism by which cytosine (C) is selected at position 75 of tRNA has been established, the mechanism by which adenine (A) is selected at position 76 remains elusive. Here, we report five cocrystal structures of the enzyme complexed with both a tRNA mimic and nucleoside triphosphates under catalytically active conditions. These structures suggest that adenosine 5'-monophosphate is incorporated onto the A76 position of the tRNA via a carboxylate-assisted, one-metal-ion mechanism with aspartate 110 functioning as a general base. The discrimination against incorporation of cytidine 5'-triphosphate (CTP) at position 76 arises from improper placement of the alpha phosphate of the incoming CTP, which results from the interaction of C with arginine 224 and prevents the nucleophilic attack by the 3' hydroxyl group of cytidine75.
-==About this Structure==
+How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA.,Pan B, Xiong Y, Steitz TA Science. 2010 Nov 12;330(6006):937-40. PMID:21071662<ref>PMID:21071662</ref>
-[[3ova]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OVA OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
+*[[CCA-adding enzyme|CCA-adding enzyme]]
 *[[TRNA|TRNA]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021071662</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Archaeoglobus fulgidus]]
-[[Category: Pan, B C.]]
+[[Category: Pan, B C]]
-[[Category: Steitz, T A.]]
+[[Category: Steitz, T A]]
-[[Category: Xiong, Y.]]
+[[Category: Xiong, Y]]
 [[Category: Cca-adding]]
 [[Category: Nucleotidyltransfer]]