1j3r: Difference between revisions

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-{{STRUCTURE_1j3r|  PDB=1j3r  |  SCENE=  }}
+==Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate==
-===Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate===
+<StructureSection load='1j3r' size='340' side='right' caption='[[1j3r]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
-{{ABSTRACT_PUBMED_12560104}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1j3r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J3R FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6PG:6-PHOSPHOGLUCONIC+ACID'>6PG</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1j3p|1j3p]], [[1j3q|1j3q]]</td></tr>
+<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j3r RCSB], [http://www.ebi.ac.uk/pdbsum/1j3r PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j3/1j3r_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The gene encoding phosphoglucose isomerase was cloned from Thermococcus litoralis, and functionally expressed in Escherichia coli. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in k(cat). The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.
-==About this Structure==
+Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis.,Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:12560104<ref>PMID:12560104</ref>
-[[1j3r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3R OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
 *[[Phosphoglucoisomerase|Phosphoglucoisomerase]]
 *[[Phosphoglucose isomerase|Phosphoglucose isomerase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:012560104</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Glucose-6-phosphate isomerase]]
 [[Category: Thermococcus litoralis]]