2b99: Difference between revisions
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'''Crystal Structure of an archaeal pentameric riboflavin synthase Complex with a Substrate analog inhibitor''' | {{Structure | ||
|PDB= 2b99 |SIZE=350|CAPTION= <scene name='initialview01'>2b99</scene>, resolution 2.22Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=RDL:6,7-DIOXO-5H-8-RIBITYLAMINOLUMAZINE'>RDL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] | |||
|GENE= ribC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii]) | |||
}} | |||
'''Crystal Structure of an archaeal pentameric riboflavin synthase Complex with a Substrate analog inhibitor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2B99 is a [ | 2B99 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B99 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor: stereochemical implications., Ramsperger A, Augustin M, Schott AK, Gerhardt S, Krojer T, Eisenreich W, Illarionov B, Cushman M, Bacher A, Huber R, Fischer M, J Biol Chem. 2006 Jan 13;281(2):1224-32. Epub 2005 Nov 4. PMID:[http:// | Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor: stereochemical implications., Ramsperger A, Augustin M, Schott AK, Gerhardt S, Krojer T, Eisenreich W, Illarionov B, Cushman M, Bacher A, Huber R, Fischer M, J Biol Chem. 2006 Jan 13;281(2):1224-32. Epub 2005 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16272154 16272154] | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Riboflavin synthase]] | [[Category: Riboflavin synthase]] | ||
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[[Category: lumazine riboflavin]] | [[Category: lumazine riboflavin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:59:02 2008'' | ||
Revision as of 16:59, 20 March 2008
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| , resolution 2.22Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ribC (Methanocaldococcus jannaschii) | ||||||
| Activity: | Riboflavin synthase, with EC number 2.5.1.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of an archaeal pentameric riboflavin synthase Complex with a Substrate analog inhibitor
OverviewOverview
Whereas eubacterial and eukaryotic riboflavin synthases form homotrimers, archaeal riboflavin synthases from Methanocaldococcus jannaschii and Methanothermobacter thermoautrophicus are homopentamers with sequence similarity to the 6,7-dimethyl-8-ribityllumazine synthase catalyzing the penultimate step in riboflavin biosynthesis. Recently it could be shown that the complex dismutation reaction catalyzed by the pentameric M. jannaschii riboflavin synthase generates riboflavin with the same regiochemistry as observed for trimeric riboflavin synthases. Here we present crystal structures of the pentameric riboflavin synthase from M. jannaschii and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine. The complex structure shows five active sites located between adjacent monomers of the pentamer. Each active site can accommodate two substrate analog molecules in anti-parallel orientation. The topology of the two bound ligands at the active site is well in line with the known stereochemistry of a pentacyclic adduct of 6,7-dimethyl-8-ribityllumazine that has been shown to serve as a kinetically competent intermediate. The pentacyclic intermediates of trimeric and pentameric riboflavin synthases are diastereomers.
About this StructureAbout this Structure
2B99 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor: stereochemical implications., Ramsperger A, Augustin M, Schott AK, Gerhardt S, Krojer T, Eisenreich W, Illarionov B, Cushman M, Bacher A, Huber R, Fischer M, J Biol Chem. 2006 Jan 13;281(2):1224-32. Epub 2005 Nov 4. PMID:16272154
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