4ay1: Difference between revisions
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==Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide binding properties== | |||
<StructureSection load='4ay1' size='340' side='right' caption='[[4ay1]], [[Resolution|resolution]] 1.95Å' scene=''> | |||
=== | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ay1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AY1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AY1 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ay1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ay1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ay1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ay1 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The chitinase-like proteins YKL-39 (chitinase 3-like-2) and YKL-40 (chitinase 3-like-1) are highly expressed in a number of human cells independent of their origin (mesenchymal, epithelial or haemapoietic). Elevated serum levels of YKL-40 have been associated with a negative outcome in a number of diseases ranging from cancer to inflammation and asthma. YKL-39 expression has been associated with osteoarthritis. However, despite the reported association with disease, the physiological or pathological role of these proteins is still very poorly understood. Although YKL-39 is homologous to the two family 18 chitinases in the human genome, it has been reported to lack any chitinase activity. In the present study, we show that human YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. A glycan screen identified oligomers of N-acetylglucosamine as preferred binding partners. YKL-39 binds chitooligosaccharides and a newly synthesized derivative of the bisdionin chitinase-inhibitor class with micromolar affinity, through a number of conserved tryptophan residues. Strikingly, the chitinase activity of YKL-39 was recovered by reverting two non-conservative substitutions in the active site to those found in the active enzymes, suggesting that YKL-39 is a pseudo-chitinase with retention of chitinase-like ligand-binding properties. | |||
Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties.,Schimpl M, Rush CL, Betou M, Eggleston IM, Recklies AD, van Aalten DM Biochem J. 2012 Aug 15;446(1):149-57. PMID:22742450<ref>PMID:22742450</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
< | </div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Aalten, D M.F Van | [[Category: Aalten, D M.F Van]] | ||
[[Category: Betou, M | [[Category: Betou, M]] | ||
[[Category: Eggleston, I M | [[Category: Eggleston, I M]] | ||
[[Category: Penman, G A | [[Category: Penman, G A]] | ||
[[Category: Recklies, A D | [[Category: Recklies, A D]] | ||
[[Category: Rush, C L | [[Category: Rush, C L]] | ||
[[Category: Schimpl, M | [[Category: Schimpl, M]] | ||
[[Category: Chilectin]] | [[Category: Chilectin]] | ||
[[Category: Chitooligosaccharide]] | [[Category: Chitooligosaccharide]] | ||