2azk: Difference between revisions
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[[Image:2azk.gif|left|200px]] | [[Image:2azk.gif|left|200px]] | ||
'''Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase''' | {{Structure | ||
|PDB= 2azk |SIZE=350|CAPTION= <scene name='initialview01'>2azk</scene>, resolution 2.70Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] | |||
|GENE= gdS-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]) | |||
}} | |||
'''Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AZK is a [ | 2AZK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZK OCA]. | ||
==Reference== | ==Reference== | ||
Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures., Sun HY, Ko TP, Kuo CJ, Guo RT, Chou CC, Liang PH, Wang AH, J Bacteriol. 2005 Dec;187(23):8137-48. PMID:[http:// | Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures., Sun HY, Ko TP, Kuo CJ, Guo RT, Chou CC, Liang PH, Wang AH, J Bacteriol. 2005 Dec;187(23):8137-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16291686 16291686] | ||
[[Category: Dimethylallyltranstransferase]] | [[Category: Dimethylallyltranstransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Sun, H Y.]] | [[Category: Sun, H Y.]] | ||
[[Category: Wang, A H.J.]] | [[Category: Wang, A H.J.]] | ||
[[Category: | [[Category: hexpp]] | ||
[[Category: isoprenyl pyrophosphate synthase]] | [[Category: isoprenyl pyrophosphate synthase]] | ||
[[Category: trans-prenyltransferase]] | [[Category: trans-prenyltransferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:55:45 2008'' | ||
Revision as of 16:55, 20 March 2008
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| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | gdS-2 (Sulfolobus solfataricus) | ||||||
| Activity: | Dimethylallyltranstransferase, with EC number 2.5.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase
OverviewOverview
Hexaprenyl pyrophosphate synthase (HexPPs) from Sulfolobus solfataricus catalyzes the synthesis of trans-C(30)-hexaprenyl pyrophosphate (HexPP) by reacting two isopentenyl pyrophosphate molecules with one geranylgeranyl pyrophosphate. The crystal structure of the homodimeric C(30)-HexPPs resembles those of other trans-prenyltransferases, including farnesyl pyrophosphate synthase (FPPs) and octaprenyl pyrophosphate synthase (OPPs). In both subunits, 10 core helices are arranged about a central active site cavity. Leu164 in the middle of the cavity controls the product chain length. Two protein conformers are observed in the S. solfataricus HexPPs structure, and the major difference between them occurs in the flexible region of residues 84 to 100. Several helices (alphaI, alphaJ, alphaK, and part of alphaH) and the associated loops have high-temperature factors in one monomer, which may be related to the domain motion that controls the entrance to the active site. Different side chain conformations of Trp136 in two HexPPs subunits result in weaker hydrophobic interactions at the dimer interface, in contrast to the symmetric pi-pi stacking interactions of aromatic side chains found in FPPs and OPPs. Finally, the three-conformer switched model may explain the catalytic process for HexPPs.
About this StructureAbout this Structure
2AZK is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
ReferenceReference
Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures., Sun HY, Ko TP, Kuo CJ, Guo RT, Chou CC, Liang PH, Wang AH, J Bacteriol. 2005 Dec;187(23):8137-48. PMID:16291686
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