2ayg: Difference between revisions
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[[Image:2ayg.gif|left|200px]] | [[Image:2ayg.gif|left|200px]] | ||
'''Crystal structure of HPV6a E2 DNA binding domain bound to an 18 base pair DNA target''' | {{Structure | ||
|PDB= 2ayg |SIZE=350|CAPTION= <scene name='initialview01'>2ayg</scene>, resolution 3.100Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= E2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10583 Human papillomavirus type 1a]) | |||
}} | |||
'''Crystal structure of HPV6a E2 DNA binding domain bound to an 18 base pair DNA target''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AYG is a [ | 2AYG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_1a Human papillomavirus type 1a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYG OCA]. | ||
==Reference== | ==Reference== | ||
The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein., Hooley E, Fairweather V, Clarke AR, Gaston K, Brady RL, Nucleic Acids Res. 2006;34(14):3897-908. Epub 2006 Aug 12. PMID:[http:// | The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein., Hooley E, Fairweather V, Clarke AR, Gaston K, Brady RL, Nucleic Acids Res. 2006;34(14):3897-908. Epub 2006 Aug 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16914454 16914454] | ||
[[Category: Human papillomavirus type 1a]] | [[Category: Human papillomavirus type 1a]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:55:23 2008'' | ||
Revision as of 16:55, 20 March 2008
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| , resolution 3.100Å | |||||||
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| Gene: | E2 (Human papillomavirus type 1a) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of HPV6a E2 DNA binding domain bound to an 18 base pair DNA target
OverviewOverview
The E2 proteins are transcription/replication factors from papillomaviruses. Human papillomaviruses (HPVs) can be broadly divided in two groups; low-risk HPV subtypes cause benign warts while high-risk HPVs give rise to cervical cancer. Although a range of crystal structures of E2 DNA-binding domains (DBD) from both high- and low-risk HPV subtypes have been reported previously, structures of E2 DBD:DNA complexes have only been available for high-risk HPV18 and bovine papillomavirus (BPV1). In the present study we report the unliganded and DNA complex structures of the E2 DBD from the low-risk HPV6. As in the previous E2-DNA structures, complex formation results in considerable bending of the DNA, which is facilitated by sequences with A:T-rich spacers that adopt a pre-bent conformation. The low-risk HPV6 E2-DNA complex differs from the earlier structures in that minimal deformation of the protein accompanies complex formation. Stopped-flow kinetic studies confirm that both high- and low-risk E2 proteins adapt their structures on binding to DNA, although this is achieved more readily for HPV6 E2. It therefore appears that the higher selectivity of the HPV6 E2 protein may arise from its limited molecular adaptability, a property that might distinguish the behaviour of E2 proteins from high- and low-risk HPV subtypes.
About this StructureAbout this Structure
2AYG is a Single protein structure of sequence from Human papillomavirus type 1a. Full crystallographic information is available from OCA.
ReferenceReference
The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein., Hooley E, Fairweather V, Clarke AR, Gaston K, Brady RL, Nucleic Acids Res. 2006;34(14):3897-908. Epub 2006 Aug 12. PMID:16914454
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