4h2x: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
{{STRUCTURE_4h2x| PDB=4h2x | SCENE= }} | {{STRUCTURE_4h2x| PDB=4h2x | SCENE= }} | ||
===Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate=== | ===Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate=== | ||
{{ABSTRACT_PUBMED_23541895}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/AACL1_BRAJA AACL1_BRAJA]] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [[http://www.uniprot.org/uniprot/AACP_AGRT5 AACP_AGRT5]] Aminoacyl carrier protein. Can be charged with L-alanine, L-glycine or L-serine, via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the Atu2573 ligase. | |||
==About this Structure== | ==About this Structure== | ||
| Line 11: | Line 10: | ||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID:020663952</ref><references group="xtra"/> | <ref group="xtra">PMID:020663952</ref><references group="xtra"/><references/> | ||
[[Category: Agrobacterium fabrum str. c58]] | [[Category: Agrobacterium fabrum str. c58]] | ||
[[Category: Bradyrhizobium japonicum usda 110]] | [[Category: Bradyrhizobium japonicum usda 110]] | ||
Revision as of 00:56, 11 April 2013
Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylateCrystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate
Template:ABSTRACT PUBMED 23541895
FunctionFunction
[AACL1_BRAJA] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [AACP_AGRT5] Aminoacyl carrier protein. Can be charged with L-alanine, L-glycine or L-serine, via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the Atu2573 ligase.
About this StructureAbout this Structure
4h2x is a 4 chain structure with sequence from Agrobacterium fabrum str. c58 and Bradyrhizobium japonicum usda 110. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Mocibob M, Ivic N, Bilokapic S, Maier T, Luic M, Ban N, Weygand-Durasevic I. Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis. Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14585-90. Epub 2010 Jul 27. PMID:20663952 doi:10.1073/pnas.1007470107