2ajt: Difference between revisions

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Revision as of 16:50, 20 March 2008

File:2ajt.gif

, resolution 2.60Å

Gene:

araA (Escherichia coli)

Activity:

L-arabinose isomerase, with EC number 5.3.1.4

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of L-Arabinose Isomerase from E.coli

OverviewOverview

Escherichia coli L-arabinose isomerase (ECAI; EC 5.3.1.4) catalyzes the isomerization of L-arabinose to L-ribulose in vivo. This enzyme is also of commercial interest as it catalyzes the conversion of D-galactose to D-tagatose in vitro. The crystal structure of ECAI was solved and refined at 2.6 A resolution. The subunit structure of ECAI is organised into three domains: an N-terminal, a central and a C-terminal domain. It forms a crystallographic trimeric architecture in the asymmetric unit. Packing within the crystal suggests the idea that ECAI can form a hexameric assembly. Previous electron microscopic and biochemical studies supports that ECAI is hexameric in solution. A comparison with other known structures reveals that ECAI adopts a protein fold most similar to E. coli fucose isomerase (ECFI) despite very low sequence identity 9.7%. The structural similarity between ECAI and ECFI with regard to number of domains, overall fold, biological assembly, and active site architecture strongly suggests that the enzymes have functional similarities. Further, the crystal structure of ECAI forms a basis for identifying molecular determinants responsible for isomerization of arabinose to ribulose in vivo and galactose to tagatose in vitro.

About this StructureAbout this Structure

2AJT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production., Manjasetty BA, Chance MR, J Mol Biol. 2006 Jul 7;360(2):297-309. Epub 2006 May 5. PMID:16756997

Page seeded by OCA on Thu Mar 20 15:50:33 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:2ajt.gif|left|200px]]<br /><applet load="2ajt" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ajt.gif|left|200px]]
-caption="2ajt, resolution 2.60&Aring;" />
-'''Crystal structure of L-Arabinose Isomerase from E.coli'''<br />
+ {{Structure
+|PDB= 2ajt |SIZE=350|CAPTION= <scene name='initialview01'>2ajt</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/L-arabinose_isomerase L-arabinose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.4 5.3.1.4]
+|GENE= araA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''Crystal structure of L-Arabinose Isomerase from E.coli'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/L-arabinose_isomerase L-arabinose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.4 5.3.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJT OCA].
+AJT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJT OCA].
 ==Reference==
-Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production., Manjasetty BA, Chance MR, J Mol Biol. 2006 Jul 7;360(2):297-309. Epub 2006 May 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16756997 16756997]
+Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production., Manjasetty BA, Chance MR, J Mol Biol. 2006 Jul 7;360(2):297-309. Epub 2006 May 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16756997 16756997]
 [[Category: Escherichia coli]]
 [[Category: L-arabinose isomerase]]
@@ Line 27: / Line 36: @@
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:28:08 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:50:33 2008''