2aiv: Difference between revisions
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[[Image:2aiv.gif|left|200px]] | [[Image:2aiv.gif|left|200px]] | ||
'''Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P''' | {{Structure | ||
|PDB= 2aiv |SIZE=350|CAPTION= <scene name='initialview01'>2aiv</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= NUP116, NSP116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AIV is a [ | 2AIV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AIV OCA]. | ||
==Reference== | ==Reference== | ||
Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p., Robinson MA, Park S, Sun ZY, Silver PA, Wagner G, Hogle JM, J Biol Chem. 2005 Oct 21;280(42):35723-32. Epub 2005 Aug 16. PMID:[http:// | Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p., Robinson MA, Park S, Sun ZY, Silver PA, Wagner G, Hogle JM, J Biol Chem. 2005 Oct 21;280(42):35723-32. Epub 2005 Aug 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16105837 16105837] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: beta sandwich]] | [[Category: beta sandwich]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:50:14 2008'' | ||
Revision as of 16:50, 20 March 2008
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| Gene: | NUP116, NSP116 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P
OverviewOverview
The yeast nucleoporin Nup116p plays an important role in mRNA export and protein transport. We have determined the solution structure of the C-terminal 147 residues of this protein, the region responsible for targeting the protein to the nuclear pore complex (NPC). The structure of Nup116p-C consists of a large beta-sheet sandwiched against a smaller one, flanked on both sides by alpha-helical stretches, similar to the structure of its human homolog, NUP98. In unliganded form, Nup116p-C exhibits evidence of exchange among multiple conformations, raising the intriguing possibility that it may adopt distinct conformations when bound to different partners in the NPC. We have additionally shown that a peptide from the N terminus of the nucleoporin Nup145p-C binds Nup116p-C. This previously unknown interaction may explain the unusual asymmetric localization pattern of Nup116p in the NPC. Strikingly, the exchange phenomenon observed in the unbound state is greatly reduced in the corresponding spectra of peptide-bound Nup116p-C, suggesting that the binding interaction stabilizes the domain conformation. This study offers a high resolution view of a yeast nucleoporin structural domain and may provide insights into NPC architecture and function.
About this StructureAbout this Structure
2AIV is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p., Robinson MA, Park S, Sun ZY, Silver PA, Wagner G, Hogle JM, J Biol Chem. 2005 Oct 21;280(42):35723-32. Epub 2005 Aug 16. PMID:16105837
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