2ahm: Difference between revisions

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Revision as of 16:49, 20 March 2008

File:2ahm.gif

, resolution 2.4Å

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Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer

OverviewOverview

Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). We report the crystal structure of the hexadecameric nsp7-nsp8 supercomplex from the severe acute respiratory syndrome coronavirus at 2.4-angstroms resolution. nsp8 has a novel 'golf-club' fold with two conformations. The supercomplex is a unique hollow, cylinder-like structure assembled from eight copies of nsp8 and held tightly together by eight copies of nsp7. With an internal diameter of approximately 30 angstroms, the central channel has dimensions and positive electrostatic properties favorable for nucleic acid binding, implying that its role is to confer processivity on RNA-dependent RNA polymerase.

About this StructureAbout this Structure

2AHM is a Protein complex structure of sequences from Human sars coronavirus. Full crystallographic information is available from OCA.

ReferenceReference

Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer., Zhai Y, Sun F, Li X, Pang H, Xu X, Bartlam M, Rao Z, Nat Struct Mol Biol. 2005 Nov;12(11):980-6. PMID:16228002

Page seeded by OCA on Thu Mar 20 15:49:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2ahm.gif|left|200px]]<br /><applet load="2ahm" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ahm.gif|left|200px]]
-caption="2ahm, resolution 2.4&Aring;" />
-'''Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer'''<br />
+ {{Structure
+|PDB= 2ahm |SIZE=350|CAPTION= <scene name='initialview01'>2ahm</scene>, resolution 2.4&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of SARS-CoV super complex of non-structural proteins: the hexadecamer'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AHM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHM OCA].
+AHM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHM OCA].
 ==Reference==
-Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer., Zhai Y, Sun F, Li X, Pang H, Xu X, Bartlam M, Rao Z, Nat Struct Mol Biol. 2005 Nov;12(11):980-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16228002 16228002]
+Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer., Zhai Y, Sun F, Li X, Pang H, Xu X, Bartlam M, Rao Z, Nat Struct Mol Biol. 2005 Nov;12(11):980-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16228002 16228002]
 [[Category: Human sars coronavirus]]
 [[Category: Protein complex]]
@@ Line 27: / Line 36: @@
 [[Category: super-complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:28 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:49:49 2008''