2adw: Difference between revisions
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[[Image:2adw.gif|left|200px]] | [[Image:2adw.gif|left|200px]] | ||
'''Crystal strucutre of Echinomycin-(ACGTACGT)2 solved by SAD''' | {{Structure | ||
|PDB= 2adw |SIZE=350|CAPTION= <scene name='initialview01'>2adw</scene>, resolution 1.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=QUI:2-CARBOXYQUINOXALINE'>QUI</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene> and <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC ACID'>MES</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal strucutre of Echinomycin-(ACGTACGT)2 solved by SAD''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2ADW is a [ | 2ADW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADW OCA]. | ||
==Reference== | ==Reference== | ||
Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex., Cuesta-Seijo JA, Weiss MS, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):417-24. Epub 2006, Mar 18. PMID:[http:// | Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex., Cuesta-Seijo JA, Weiss MS, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):417-24. Epub 2006, Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16552143 16552143] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cuesta-Seijo, J A.]] | [[Category: Cuesta-Seijo, J A.]] | ||
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[[Category: hoogsteen/watson-crick]] | [[Category: hoogsteen/watson-crick]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:48:28 2008'' | ||
Revision as of 16:48, 20 March 2008
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| , resolution 1.60Å | |||||||
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| Ligands: | , , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal strucutre of Echinomycin-(ACGTACGT)2 solved by SAD
OverviewOverview
A new crystal form was obtained for the complex between (ACGTACGT)2 and echinomycin and X-ray data were collected to 1.6 A. The structure was phased by the SAD method based on a single unexpected anomalous scatterer that could be identified as a mixture of nickel and zinc by measurements of the anomalous scattering at different wavelengths. This cation is coordinated by two guanines from two different duplexes and four water molecules. The structure resembles previously reported crystal structures of DNA-echinomycin complexes, except that three of the eight base pairs flanking the echinomycin bisintercalator sites have the Watson-Crick rather than the Hoogsteen configuration. Hoogsteen binding was found for the corresponding base pairs of the crystallographically independent duplex, indicating that the two configurations are very close in energy.
About this StructureAbout this Structure
2ADW is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex., Cuesta-Seijo JA, Weiss MS, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):417-24. Epub 2006, Mar 18. PMID:16552143
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