2a97: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2a97.gif|left|200px]] | [[Image:2a97.gif|left|200px]] | ||
'''Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F''' | {{Structure | ||
|PDB= 2a97 |SIZE=350|CAPTION= <scene name='initialview01'>2a97</scene>, resolution 1.80Å | |||
|SITE= <scene name='pdbsite=CDA:First+Cd+Binding+Site'>CDA</scene>, <scene name='pdbsite=CDB:Second+Cd+Binding+Site'>CDB</scene>, <scene name='pdbsite=CDC:Third+Cd+Binding+Site'>CDC</scene>, <scene name='pdbsite=CDD:Fourt+Cd+Binding+Site'>CDD</scene>, <scene name='pdbsite=CDE:Fifth+Cd+Binding+Site'>CDE</scene>, <scene name='pdbsite=CDF:Sixth+Cd+Binding+Site'>CDF</scene>, <scene name='pdbsite=ZNA:First+Zn+Binding+Site'>ZNA</scene> and <scene name='pdbsite=ZNB:Second+Zn+Binding+Site'>ZNB</scene> | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CD:CADMIUM ION'>CD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] | |||
|GENE= botF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum]) | |||
}} | |||
'''Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2A97 is a [ | 2A97 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A97 OCA]. | ||
==Reference== | ==Reference== | ||
Structural analysis of botulinum neurotoxin serotype F light chain: implications on substrate binding and inhibitor design., Agarwal R, Binz T, Swaminathan S, Biochemistry. 2005 Sep 6;44(35):11758-65. PMID:[http:// | Structural analysis of botulinum neurotoxin serotype F light chain: implications on substrate binding and inhibitor design., Agarwal R, Binz T, Swaminathan S, Biochemistry. 2005 Sep 6;44(35):11758-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16128577 16128577] | ||
[[Category: Bontoxilysin]] | [[Category: Bontoxilysin]] | ||
[[Category: Clostridium botulinum]] | [[Category: Clostridium botulinum]] | ||
| Line 21: | Line 30: | ||
[[Category: clostridium botulinum neurotoxin serotype f; light chain; catalytic domain; x-ray; crystal structure; zinc endopeptidase]] | [[Category: clostridium botulinum neurotoxin serotype f; light chain; catalytic domain; x-ray; crystal structure; zinc endopeptidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:46:58 2008'' | ||
Revision as of 16:46, 20 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , and | ||||||
| Ligands: | and | ||||||
| Gene: | botF (Clostridium botulinum) | ||||||
| Activity: | Bontoxilysin, with EC number 3.4.24.69 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F
OverviewOverview
The seven serologically distinct Clostridium botulinum neurotoxins (BoNTs A-G) are zinc endopeptidases which block the neurotransmitter release by cleaving one of the three proteins of the soluble N-ethylmaleimide-sensitive-factor attachment protein receptor complex (SNARE complex) essential for the fusion of vesicles containing neurotransmitters with target membranes. These metallopeptidases exhibit unique specificity for the substrates and peptide bonds they cleave. Development of countermeasures and therapeutics for BoNTs is a priority because of their extreme toxicity and potential misuse as biowarfare agents. Though they share sequence homology and structural similarity, the structural information on each one of them is required to understand the mechanism of action of all of them because of their specificity. Unraveling the mechanism will help in the ultimate goal of developing inhibitors as antibotulinum drugs for the toxins. Here, we report the high-resolution structure of active BoNT/F catalytic domain in two crystal forms. The structure was exploited for modeling the substrate binding and identifying the S1' subsite and the putative exosites which are different from BoNT/A or BoNT/B. The orientation of docking of the substrate at the active site is consistent with the experimental BoNT/A-LC:SNAP-25 peptide model and our proposed model for BoNT/E-LC:SNAP-25.
About this StructureAbout this Structure
2A97 is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of botulinum neurotoxin serotype F light chain: implications on substrate binding and inhibitor design., Agarwal R, Binz T, Swaminathan S, Biochemistry. 2005 Sep 6;44(35):11758-65. PMID:16128577
Page seeded by OCA on Thu Mar 20 15:46:58 2008