3sbt: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3sbt.png|left|200px]]
+==Crystal structure of a Aar2-Prp8 complex==
+<StructureSection load='3sbt' size='340' side='right' caption='[[3sbt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3sbt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SBT FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3e9o|3e9o]], [[3sbs|3sbs]], [[3sbg|3sbg]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), AAR2, YBL06.06, YBL0611, YBL074C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sbt RCSB], [http://www.ebi.ac.uk/pdbsum/3sbt PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Little is known about how particle-specific proteins are assembled on spliceosomal small nuclear ribonucleoproteins (snRNPs). Brr2p is a U5 snRNP-specific RNA helicase required for spliceosome catalytic activation and disassembly. In yeast, the Aar2 protein is part of a cytoplasmic precursor U5 snRNP that lacks Brr2p and is replaced by Brr2p in the nucleus. Here we show that Aar2p and Brr2p bind to different domains in the C-terminal region of Prp8p; Aar2p interacts with the RNaseH domain, whereas Brr2p interacts with the Jab1/MPN domain. These domains are connected by a long, flexible linker, but the Aar2p-RNaseH complex sequesters the Jab1/MPN domain, thereby preventing binding by Brr2p. Aar2p is phosphorylated in vivo, and a phospho-mimetic S253E mutation in Aar2p leads to disruption of the Aar2p-Prp8p complex in favor of the Brr2p-Prp8p complex. We propose a model in which Aar2p acts as a phosphorylation-controlled U5 snRNP assembly factor that regulates the incorporation of the particle-specific Brr2p. The purpose of this regulation may be to safeguard against nonspecific RNA binding to Prp8p and/or premature activation of Brr2p activity.
-{{STRUCTURE_3sbt|  PDB=3sbt  |  SCENE=  }}
+Mechanism for Aar2p function as a U5 snRNP assembly factor.,Weber G, Cristao VF, Alves FD, Santos KF, Holton N, Rappsilber J, Beggs JD, Wahl MC Genes Dev. 2011 Jul 15. PMID:21764848<ref>PMID:21764848</ref>
-===Crystal structure of a Aar2-Prp8 complex===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_21764848}}
-==About this Structure==
-[[3sbt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBT OCA].
 ==See Also==
 *[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021764848</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Saccharomyces cerevisiae s288c]]
-[[Category: Holton, N.]]
+[[Category: Holton, N]]
-[[Category: Santos, K F.]]
+[[Category: Santos, K F]]
-[[Category: Wahl, M C.]]
+[[Category: Wahl, M C]]
-[[Category: Weber, G.]]
+[[Category: Weber, G]]
 [[Category: Rnaseh like domain]]
 [[Category: Splicing]]
 [[Category: U5 snrnp assembly]]
 [[Category: Vhs like domain]]