3fc0: Difference between revisions

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-[[Image:3fc0.png|left|200px]]
+==1.8 A crystal structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells==
+<StructureSection load='3fc0' size='340' side='right' caption='[[3fc0]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3fc0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FC0 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qdn|2qdn]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tnfsf18, Gitrl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/3fc0 PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/3fc0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Glucocorticoid-induced TNF receptor ligand (GITRL), a prominent member of the TNF superfamily, activates its receptor on both effector and regulatory T cells to generate critical costimulatory signals that have been implicated in a wide range of T-cell immune functions. The crystal structures of murine and human orthologs of GITRL recombinantly expressed in Escherichia coli have previously been determined. In contrast to all classical TNF structures, including the human GITRL structure, murine GITRL demonstrated a unique ;strand-exchanged' dimeric organization. Such a novel assembly behavior indicated a dramatic impact on receptor activation as well as on the signaling mechanism associated with the murine GITRL costimulatory system. In this present work, the 1.8 A resolution crystal structure of murine GITRL expressed in Drosophila melanogaster S2 cells is reported. The eukaryotic protein-expression system allows transport of the recombinant protein into the extracellular culture medium, thus maximizing the possibility of obtaining correctly folded material devoid of any folding/assembly artifacts that are often suspected with E. coli-expressed proteins. The S2 cell-expressed murine GITRL adopts an identical ;strand-exchanged' dimeric structure to that observed for the E. coli-expressed protein, thus conclusively demonstrating the novel quaternary structure assembly behavior of murine GITRL.
-{{STRUCTURE_3fc0|  PDB=3fc0  |  SCENE=  }}
+.8 A structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells.,Chattopadhyay K, Ramagopal UA, Nathenson SG, Almo SC Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):434-9. Epub 2009, Apr 18. PMID:19390148<ref>PMID:19390148</ref>
-===1.8 A crystal structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19390148}}
+==See Also==
+*[[Tumor necrosis factor receptor|Tumor necrosis factor receptor]]
-==About this Structure==
+== References ==
-[[3fc0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FC0 OCA].
+<references/>
+__TOC__
-==Reference==
+</StructureSection>
-<ref group="xtra">PMID:019390148</ref><references group="xtra"/>
 [[Category: Mus musculus]]
-[[Category: Almo, S C.]]
+[[Category: Almo, S C]]
-[[Category: Chattopadhyay, K.]]
+[[Category: Chattopadhyay, K]]
-[[Category: Nathenson, S G.]]
+[[Category: Nathenson, S G]]
-[[Category: Ramagopal, U A.]]
+[[Category: Ramagopal, U A]]
 [[Category: Gitrl]]
 [[Category: Glucocorticoid-induced tnf receptor ligand]]
 [[Category: Protein binding]]
 [[Category: Receptor]]